Alteration in the gastric microbiota and its restoration by probiotics in patients with functional dyspepsia.
Alteration in the gastric microbiota and its restoration by probiotics in patients with functional dyspepsia.
复制标题
DOI：
10.1136/bmjgast-2017-000144
复制
发表时间：
2017
影响因子：
3.1
通讯作者：
Koga Y
中科院分区：
文献类型：
--
作者：
Igarashi M;Nakae H;Matsuoka T;Takahashi S;Hisada T;Tomita J;Koga Y
本研究的目的是比较分析功能性消化不良（FD）患者和健康对照（HC）之间的胃液（GF）微生物群，并评估益生菌对微生物群的影响。入组了24例符合罗马III定义的FD日本患者和21例年龄和性别匹配的HC志愿者。FD患者接受了益生菌LG 21的治疗。在禁食过夜后使用鼻胃管对GF进行取样。用ELISA法测定胆汁酸浓度。利用细菌DNA扩增16 SrRNA基因的V3-V4区，将每个样品约30 000个高质量的扩增子分成可操作的分类单位进行分析。 FD组中可检测到胆汁酸的GF样本比例显著高于HC组。在门水平的细菌组成分析中，GF微生物群具有拟杆菌门>变形菌门丰度，并且在FD组中不存在酸性细菌，相反，GF微生物群具有拟杆菌门<变形菌门丰度，并且在HC组中存在酸性细菌。FD患者的益生菌治疗将GF微生物群的组成转移到HC志愿者中观察到的组成。与HC志愿者相比，FD患者的GF微生物群发生了变化。包括胆汁酸和肠道细菌在内的小肠内容物回流到胃中，可能会引起细菌组成的变化，并参与FD的病理生理学。益生菌似乎通过胃微生物群的正常化有效治疗FD。UMINCTR 000022026;结果。
The objective of this study was to comparatively analyse the gastric fluid (GF) microbiota between patients with functional dyspepsia (FD) and healthy controls (HC), and to assess the effect of probiotics on the microbiota. Twenty-four Japanese patients with FD who met the Rome III definition and 21 age-matched and gender-matched HC volunteers were enrolled. The patients with FD had been treated with LG21, a probiotic strain. The GF was sampled after an overnight fast using a nasogastric tube. The bile acids concentration was determined by ELISA. The V3-V4 region of 16S rRNA gene was amplified using bacterial DNA from the GF, and then about 30 000 high-quality amplicons per sample were grouped into operational taxonomic units for analyses. The ratio of GF samples in which the bile acids were detectable was significantly greater in the FD than in the HC groups. In the bacterial composition analysis at the phylum level, the GF microbiota had a Bacteroidetes > Proteobacteria abundance and an absence of Acidobacteria in the FD group, in contrast, the GF microbiota had a Bacteroidetes < Proteobacteria abundance and the presence of Acidobacteria in the HC group. Probiotic therapy in patients with FD shifted the composition of the GF microbiota to that observed in the HC volunteers. Alteration in the GF microbiota was found in patients with FD compared with HC volunteers. Reflux of the small intestinal contents, including bile acid and intestinal bacteria, to the stomach was suggested to induce a bacterial composition change and be involved in the pathophysiology underlying FD. Probiotics appear effective in the treatment of FD through the normalisation of gastric microbiota. UMINCTR 000022026; Results.
登录
查看更多内容
影响因子：
3.7
作者：
Takahashi S;Tomita J;Nishioka K;Hisada T;Nishijima M
通讯作者：
Nishijima M
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob
DOI：
10.1111/j.1432-1033.1985.tb08798.x
发表时间：
1985-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
GALANOS, C;LUDERITZ, O;SHIBA, T
通讯作者：
SHIBA, T
影响因子：
4.3
作者：
Bures, Jan;Cyrany, Jiri;Kopacova, Marcela
通讯作者：
Kopacova, Marcela
影响因子：
24.5
作者：
Vanheel, Hanne;Vicario, Maria;Farre, Ricard
通讯作者：
Farre, Ricard