CHARACTERIZATION OF CD34+ PERIPHERAL BLOOD CELLS FROM HEALTHY ADULTS MOBILIZED BY RECOMBINANT HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR
CHARACTERIZATION OF CD34+ PERIPHERAL BLOOD CELLS FROM HEALTHY ADULTS MOBILIZED BY RECOMBINANT HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR
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重组人粒细胞集落刺激因子动员的健康成人 CD34 外周血细胞的表征
DOI：
10.1182/blood.v84.8.2795.2795
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发表时间：
1994
期刊：
影响因子：
20.3
通讯作者：
T. Egeland
中科院分区：
文献类型：
--
作者：
G. Tjønnfjord;R. Steen;S. Evensen;E. Thorsby;T. Egeland
预处理的外周血造血干细胞 (PBSC) 在清髓动物中产生并维持淋巴造血作用，最近的报告表明，使用 PBSC 移植物进行同种异体移植在人类中可能是可行的。使用 PBSC 移植的一个主要问题是，由于移植物中缺乏足够数量的原始祖细胞，永久植入可能受到限制。在本研究中，将短期粒细胞集落刺激因子（G-CSF）给药期间健康成人PB中CD34+细胞的体外集落形成和免疫表型与正常骨髓（BM）中CD34+细胞的体外集落形成和免疫表型进行了比较。动员至 PB 的 CD34+ 细胞数量在 G-CSF 施用的第 4 或 5 天达到峰值。与 CD34+ BM 细胞相比，CD34+ PB 细胞的表型特征显示，CD34+CD13+ 和 CD34+CD33+ 细胞（骨髓祖细胞）的百分比显着增加，而 CD34+CD10+ 和 CD34+CD19+ 细胞（B 淋巴祖细胞）的百分比相应下降。研究的其他亚群，包括 CD34+CD38- 和 CD34+HLA-DR- 细胞，以相似的比例存在于两个区室中。此外，与 BM 中的对应细胞相比，引发的 CD34+ PB 细胞富集集落形成细胞 (CFC)，并显示出增加的集落形成性。比较了假定的 PBSC 移植物和普通 BM 移植物，表明这种 PBSC 移植物将富集整个 CD34+ 细胞、CD34+CD33+ 细胞和集落形成细胞 (CFC)，这些因素已被证明与加速血液重建和减少自体移植中支持护理的需求相关。因此，我们预测使用 G-CSF 引发的 PBSC 移植物进行同种异体移植将比 BM 移植在清髓性调节后导致更快的血液学重建。 PBSC 同种异体移植是否会产生自体移植中观察到的永久植入和临床益处的问题必须在前瞻性临床研究中确定。
Primed peripheral blood hematopoietic stem cells (PBSC) generate and sustain lymphohematopoiesis in myeloablated animals, and recent reports indicate that allogeneic transplantation using PBSC grafts may be feasible in humans. A major concern with the use of PBSC transplants is that permanent engraftment may be limited because of lack of sufficient numbers of primitive progenitor cells in the graft. In the present study, in vitro colony formation and immunophenotype of CD34+ cells in PB of healthy adults during short-term granulocyte colony-stimulating factor (G-CSF) administration were compared with that of CD34+ cells in normal bone marrow (BM). The number of CD34+ cells mobilized to PB peaked at day 4 or 5 of G-CSF administration. The phenotypic profile of CD34+ PB cells showed a substantial increase in the percentage of CD34+CD13+ and CD34+CD33+ cells (myeloid progenitors) and a corresponding decrease in the percentage of CD34+CD10+ and CD34+CD19+ cells (B lymphoid progenitors) compared with CD34+ BM cells. The other subsets studied, including CD34+CD38- and CD34+HLA-DR- cells, were present in both compartments in similar proportions. Furthermore, primed CD34+ PB cells were enriched for colony-forming cells (CFC) and displayed an increased clonogenicity when compared with their counterparts in BM. A comparison between a postulated PBSC graft and an average BM graft is presented, showing that such PBSC grafts will be enriched for CD34+ cells as a whole, CD34+CD33+ cells, and colony- forming cells (CFC), factors which have been shown to correlate to acceleration of hematologic reconstitution and reduction in requirements for supportive care in autografting. Hence, we predict that allogeneic transplantation using G-CSF-primed PBSC grafts will result in a more rapid hematologic reconstitution after myeloablative conditioning than BM grafting. The question of whether PBSC allografting will result in permanent engraftment and clinical benefits as observed in autografting has to be determined in prospective clinical studies.
人类多谱系、自我更新的淋巴造血干细胞在嵌合羊中的持久性。
影响因子：
20.3
作者：
Srour,EF;Zanjani,ED;Cornetta,K;Traycoff,CM;Flake,AW;Hedrick,M;Brandt,JE;Leemhuis,T;Hoffman,R
通讯作者：
Hoffman,R
影响因子：
20.3
作者：
Weaver Ch;Buckner Cd;K. Longin;F. Appelbaum;S. Rowley;K. Lilleby;J. Miser;R. Storb;J. Hansen;W. Bensinger
通讯作者：
Weaver Ch;Buckner Cd;K. Longin;F. Appelbaum;S. Rowley;K. Lilleby;J. Miser;R. Storb;J. Hansen;W. Bensinger