Neuron-astrocyte interactions in the medial nucleus of the trapezoid body.
Neuron-astrocyte interactions in the medial nucleus of the trapezoid body.
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DOI：
10.1085/jgp.200910354
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发表时间：
2010-06
期刊：
影响因子：
--
通讯作者：
Kettenmann H
中科院分区：
文献类型：
--
作者：
Reyes-Haro D;Müller J;Boresch M;Pivneva T;Benedetti B;Scheller A;Nolte C;Kettenmann H
Held (CoH) 突触花萼作为模型系统来分析突触传递的基本机制。星形胶质细胞突起是突触结构的一部分，接触突触前膜和突触后膜。在梯形体内侧核（MNTB）中，中线刺激引起的电流反应不是由谷氨酸受体或谷氨酸摄取介导的，尽管星形胶质细胞表达功能性受体和转运蛋白。然而，星形胶质细胞表现出自发的 Ca2+ 反应，并且在 MNTB 的突触后主神经元 (PPN) 中记录到神经元慢内向电流 (nSIC)。这些电流与星形胶质细胞 Ca2+ 活性相关，因为用 BAPTA 透析星形胶质细胞消除了 nSIC。此外，当星形胶质细胞中引起 Ca2+ 反应时，这些电流的频率会增加。 NMDA 拮抗剂选择性阻断 nSIC，而 D-丝氨酸降解则显着降低 NMDA 介导的电流。与之前的海马体研究相比，这些 NMDA 介导的电流很少同步。
The calyx of Held (CoH) synapse serves as a model system to analyze basic mechanisms of synaptic transmission. Astrocyte processes are part of the synaptic structure and contact both pre- and postsynaptic membranes. In the medial nucleus of the trapezoid body (MNTB), midline stimulation evoked a current response that was not mediated by glutamate receptors or glutamate uptake, despite the fact that astrocytes express functional receptors and transporters. However, astrocytes showed spontaneous Ca2+ responses and neuronal slow inward currents (nSICs) were recorded in the postsynaptic principal neurons (PPNs) of the MNTB. These currents were correlated with astrocytic Ca2+ activity because dialysis of astrocytes with BAPTA abolished nSICs. Moreover, the frequency of these currents was increased when Ca2+ responses in astrocytes were elicited. NMDA antagonists selectively blocked nSICs while D-serine degradation significantly reduced NMDA-mediated currents. In contrast to previous studies in the hippocampus, these NMDA-mediated currents were rarely synchronized.
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影响因子：
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