Acetal-derivatized dextran: an acid-responsive biodegradable material for therapeutic applications.
Acetal-derivatized dextran: an acid-responsive biodegradable material for therapeutic applications.
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DOI：
10.1021/ja803947s
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发表时间：
2008-08-13
影响因子：
15
通讯作者：
Frechet, Jean M. J.
中科院分区：
文献类型：
--
作者：
Bachelder, Eric M.;Beaudette, Tristan T.;Broaders, Kyle E.;Dashe, Jesse;Frechet, Jean M. J.
右旋糖酐是一种生物相容性水溶性多糖，其羟基被缩醛部分修饰，使其不溶于水，但易溶于常见有机溶剂，使其可用于轻松制备酸敏感微粒。这些颗粒以 pH 依赖性方式降解：FITC-葡聚糖在 37 ℃、pH 5.0 下的半衰期为 10 小时，而在 pH 7.4 下的半衰期约为 15 天。分别使用单乳液和双乳液技术将疏水性和亲水性货物成功加载到这些颗粒中。当用于模型疫苗应用时，负载卵清蛋白 (OVA) 的颗粒将 OVA 衍生肽向 CD8+ T 细胞的呈递增加，相对于单独的 OVA 增加了 16 倍。此外，在初步的体外细胞毒性测定中发现这种葡聚糖衍生物是无毒的。由于其易于制备、可加工、pH 敏感性和生物相容性，这种类型的修饰葡聚糖应该在许多药物输送应用中得到应用。
Dextran, a biocompatible, water-soluble polysaccharide, was modified at its hydroxyls with acetal moieties such that it became insoluble in water but freely soluble in common organic solvents enabling its use in the facile preparation of acid-sensitive microparticles. These particles degrade in a pH-dependent manner: FITC-dextran was released with a half-life at 37 ºC of 10 hours at pH 5.0 compared to a half-life of approximately 15 days at pH 7.4. Both hydrophobic and hydrophilic cargoes were successfully loaded into these particles using single and double emulsion techniques, respectively. When used in a model vaccine application, particles loaded with the protein ovalbumin (OVA) increased the presentation of OVA-derived peptides to CD8+ T-cells 16-fold relative to OVA alone. Additionally, this dextran derivative was found to be non-toxic in preliminary in vitro cytotoxicity assays. Due to its ease of preparation, processability, pH-sensitivity, and biocompatibility, this type of modified dextran should find use in numerous drug delivery applications.
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影响因子：
3.8
作者：
YOLLES, S;LEAFE, TD;MEYER, FJ
通讯作者：
MEYER, FJ
DOI：
10.1111/j.1749-6632.1985.tb18390.x
发表时间：
1985-06-21
影响因子：
5.2
作者：
HELLER, J
通讯作者：
HELLER, J
影响因子：
10.8
作者：
Little, SR;Lynn, DM;Langer, R
通讯作者：
Langer, R
影响因子：
64.8
作者：
Sengupta, S;Eavarone, D;Sasisekharan, R
通讯作者：
Sasisekharan, R
影响因子：
4.7
作者：
Sawant, R. M.;Hurley, J. P.;Torchilin, V. P.
通讯作者：
Torchilin, V. P.