Physical and functional interactions between the transcription factor PU.1 and the coactivator CBP
Physical and functional interactions between the transcription factor PU.1 and the coactivator CBP
复制标题
转录因子 PU.1 和共激活因子 CBP 之间的物理和功能相互作用
作者：
Hitomi Yamamoto;F. Kihara;Toshiyuki Yamada;Y. Hashimoto;T. Oikawa
利用酵母双杂交系统分离与Ets家族转录因子PU. 1相互作用的新蛋白。对几个分离的β-半乳糖苷酶活性阳性克隆的序列分析显示，这些克隆中的一个被证实编码转录辅激活因子CREB结合蛋白（CBP）。GST结合实验表明，相互作用位点位于PU.1的转录激活区74 - 122和CBP的1283 - 1915残基之间。CBP增强了PU.1介导的由多聚化PU.1结合位点驱动的报告基因的转录，表明CBP作为PU.1的共激活剂发挥作用。考虑到CBP是一种有限的细胞组分，作为几种转录因子的共激活因子，CBP可能在造血细胞分化过程中介导PU. 1与其他转录因子之间的协同和拮抗相互作用。
Yeast two-hybrid system was employed to isolate novel proteins that physically interact with PU.1, a member of Ets family transcription factors. Sequence analyses of several isolated clones positive for β-galactosidase activity revealed that one of these clones was confirmed to encode a transcriptional coactivator, CREB binding protein (CBP). GST binding assay showed that the interacting sites were located at the transcriptional activation domain of PU.1 through 74 – 122 and the region spanning residues 1283 – 1915 of CBP. CBP potentiated PU.1-mediated transcription of the reporter gene driven by the multimerized PU.1-binding sites, suggesting that CBP functions as a coactivator for PU.1. Considering that CBP is a limited cellular component to function as a coactivator for several transcription factors, CBP may mediate synergistic and antagonistic interactions between PU.1 and other transcription factors during the process of hematopoietic cell differentiation.
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影响因子：
56.9
作者：
SCOTT, EW;SIMON, MC;SINGH, H
通讯作者：
SINGH, H
影响因子：
20.3
作者：
Bellon, T;Perrotti, D;Calabretta, B
通讯作者：
Calabretta, B
影响因子：
56.9
作者：
PONGUBALA, JMR;VANBEVEREN, C;ATCHISON, ML
通讯作者：
ATCHISON, ML
DOI：
10.1073/pnas.94.7.2927
发表时间：
1997-04-01
影响因子：
11.1
作者：
Gerritsen, ME;Williams, AJ;Collins, T
通讯作者：
Collins, T
影响因子：
11.4
作者：
McKercher, SR;Torbett, BE;Maki, RA
通讯作者：
Maki, RA