SRPK1 Dissimilarly Impacts on the Growth, Metastasis, Chemosensitivity and Angiogenesis of Glioma in Normoxic and Hypoxic Conditions.
SRPK1 Dissimilarly Impacts on the Growth, Metastasis, Chemosensitivity and Angiogenesis of Glioma in Normoxic and Hypoxic Conditions.
复制标题
DOI：
10.7150/jca.7576
复制
发表时间：
2013
影响因子：
3.9
通讯作者：
Huang F
中科院分区：
文献类型：
--
作者：
Wu Q;Chang Y;Zhang L;Zhang Y;Tian T;Feng G;Zhou S;Zheng Q;Han F;Huang F
胶质瘤是癌症相关死亡的十大最常见原因之一，并且没有有效的治疗方法，因此我们正在努力寻找抗癌治疗的新靶点。本研究旨在探讨SRPK 1作为一种新的蛋白质在脑胶质瘤中的表达差异，并探讨其在常氧和低氧环境中对肿瘤细胞生物学特性的影响。分别用免疫荧光、RT-PCR和Western blot检测转染和未转染SRPK 1的胶质瘤细胞中SRPK 1蛋白的表达水平。采用MTT法、流式细胞术和Transwell侵袭小室法进一步研究SRPK 1对U251细胞生物学特性的影响。结果表明，在常氧条件下，SRPK 1基因敲低可抑制肿瘤细胞的生长、侵袭和迁移，但在低氧条件下，这种作用可部分逆转。在常氧和缺氧条件下，DDP处理的胶质瘤细胞诱导SRPK 1表达，而TMZ则不诱导。我们建议SRPK 1作为一个新的分子球员有助于胶质瘤的早期治疗。
Glioma is among the ten most common causes of cancer-related death and has no effective treatment for it, so we are trying to find a new target for anticancer treatment. This study investigates the different expression of SRPK1 as a novel protein in glioma, which can influence tumor cells biological characteristics in normoxic and hypoxic environment. The expression levels of SRPK1 protein in glioma cell lines transfected with siSRPK1 or not were examined using immunofluorescence, RT-PCR and Western blot analysis, respectively. The impact of SRPK1 on the biological characteristics of U251 cells was further studied using methylthiazol tetrazolium assays, flow cytometry, and Transwell invasion chamber assays. The results showed that knockdown of SRPK1 inhibited tumor cells growth, invasion and migration in normoxic condition, but portion of the effect could be reversed in hypoxia. SRPK1 expression was induced in glioma cells by DDP treated, but not TMZ, in both normoxia and hypoxia conditions. We propose SRPK1 as a new molecular player contributing to the early treatment of glioma.
登录
查看更多内容
影响因子：
3.2
作者：
Krishnakumar, Subramanian;Mohan, Adithi;Das, Radha R.
通讯作者：
Das, Radha R.
影响因子：
10.4
作者：
Mandal, SK;Rao, LVM;Pendurthi, JR
通讯作者：
Pendurthi, JR
影响因子：
5.5
作者：
Oda, Tomoyuki;Hirota, Kiichi;Nohara, Ryuji
通讯作者：
Nohara, Ryuji
影响因子：
4
作者：
Cassavaugh, Jessica;Lounsbury, Karen M.
通讯作者：
Lounsbury, Karen M.
影响因子：
14.9
作者：
Papoutsopoulou, S;Nikolakaki, E;Giannakouros, T
通讯作者：
Giannakouros, T