A new strategy for methylated DNA detection based on photoelectrochemical immunosensor using Bi2S3 nanorods, methyl bonding domain protein and anti-his tag antibody.
A new strategy for methylated DNA detection based on photoelectrochemical immunosensor using Bi2S3 nanorods, methyl bonding domain protein and anti-his tag antibody.
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基于光电化学免疫传感器的甲基化DNA检测新策略，使用Bi2S3纳米棒、甲基键合域蛋白和抗his标签抗体。
DOI：
10.1016/j.bios.2013.07.040
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发表时间：
2014-01
影响因子：
12.6
通讯作者：
Ai, Shiyun
中科院分区：
文献类型：
--
作者：
Yin, Huanshun;Sun, Bing;Zhou, Yunlei;Wang,Mo;Xu, Zhenning;Fu, Zhengliang;Ai, Shiyun
本文以bi2s3纳米棒为光电转换材料，以甲基结合结构域蛋白MBD1为DNA甲基化识别单元，利用抗his标记抗体进一步抑制光电流，提高检测灵敏度，制备了一种新型的DNA甲基化检测电化学免疫传感器。结果表明，bi2s具有优异的光电子性能。优化检测条件，如bi2o3浓度、MBD1蛋白浓度、MBD1蛋白孵育时间、抗体浓度和抗体孵育时间。在最佳实验条件下，光电流变化与甲基化目标DNA浓度的对数成正比，范围为10−9 ~ 10 - 13m，检出限为3.5×10-14M （S/N=3）。此外，该免疫传感器具有较高的检测特异性，甚至可以区分单碱基错配序列。
In this work, we fabricated a novel photoelectrochemical immunosensor for assay of DNA methylation, where Bi2S3nanorods were used as photoelectric conversion material, MBD1 protein (a kind of methyl bonding domain protein) was used as DNA methylation recognizing unit, anti-his tag antibody was used to further inhibit the photocurrent and increase the detection sensitivity. The results demonstrated that Bi2S3possessed excellent photoelectron property. The detection conditions, such as Bi2S3concentration, MBD1 protein concentration, incubation time of MBD1 protein, antibody concentration and antibody incubation time, were optimized. Under optimal experimental conditions, the photocurrent variation was proportional to the logarithm of methylated target DNA concentration from 10−9to 10–13M with detection limit of 3.5×10–14M (S/N=3). Moreover, the immunosensor presented high detection specificity, even distinguishing single-base mismatched sequence.
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影响因子：
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DOI：
10.1039/c2an35043b
发表时间：
2012-04
期刊：
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影响因子：
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