Probiotic L. reuteri treatment prevents bone loss in a menopausal ovariectomized mouse model.
Probiotic L. reuteri treatment prevents bone loss in a menopausal ovariectomized mouse model.
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DOI：
10.1002/jcp.24636
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发表时间：
2014-11
影响因子：
5.6
通讯作者：
McCabe, Laura R.
中科院分区：
文献类型：
--
作者：
Britton, Robert A.;Irwin, Regina;Quach, Darin;Schaefer, Laura;Zhang, Jing;Lee, Taehyung;Parameswaran, Narayanan;McCabe, Laura R.
雌激素缺乏是骨质疏松症的主要危险因素，与骨炎症和吸收有关。 50 岁以上的女性中有一半会在一生中经历骨质疏松症相关的骨折，因此需要新的疗法来对抗绝经后骨质流失。最近的研究表明肠骨信号通路和微生物群在调节骨骼健康中发挥着重要作用。鉴于罗伊氏乳杆菌 ATCC PTA 6475 (L. reuteri) 分泌有益的免疫调节因子，我们研究了这种候选益生菌是否可以减少卵巢切除 (Ovx) 小鼠绝经模型中与雌激素缺乏相关的骨质流失。引人注目的是，罗伊氏乳杆菌治疗显着保护 Ovx 小鼠免受骨质流失。罗伊氏乳杆菌治疗的小鼠中，破骨细胞骨吸收标记物和激活剂（Trap5 和 RANKL）以及破骨细胞生成显着减少。与此一致的是，罗伊氏乳杆菌抑制了 Ovx 诱导的骨髓 CD4+ T 淋巴细胞（促进破骨细胞生成）的增加，并在体外直接抑制破骨细胞生成。我们还发现罗伊氏乳杆菌治疗改变了 Ovx 小鼠肠道中的微生物群落。总之，我们的研究表明，罗伊氏乳杆菌治疗可抑制与雌激素缺乏相关的骨吸收和损失。因此，罗伊氏乳杆菌治疗可能是减少绝经后骨质流失的一种直接且经济有效的方法。
Estrogen deficiency is a major risk factor for osteoporosis that is associated with bone inflammation and resorption. Half of women over the age of 50 will experience an osteoporosis related fracture in their lifetime, thus novel therapies are needed to combat post-menopausal bone loss. Recent studies suggest an important role for gut-bone signaling pathways and the microbiota in regulating bone health. Given that the bacterium Lactobacillus reuteri ATCC PTA 6475 (L. reuteri) secretes beneficial immunomodulatory factors, we examined if this candidate probiotic could reduce bone loss associated with estrogen deficiency in an ovariectomized (Ovx) mouse menopausal model. Strikingly, L. reuteri treatment significantly protected Ovx mice from bone loss. Osteoclast bone resorption markers and activators (Trap5 and RANKL) as well as osteoclastogenesis are significantly decreased in L. reuteri treated mice. Consistent with this, L. reuteri suppressed Ovx-induced increases in bone marrow CD4+ T-lymphocytes (which promote osteoclastogenesis) and directly suppressed osteoclastogenesis in vitro. We also identif ied that L. reuteri treatment modifies microbial communities in the Ovx mouse gut. Together, our studies demonstrate that L. reuteri treatment suppresses bone resorption and loss associated with estrogen deficiency. Thus, L. reuteri treatment may be a straightforward and cost-effective approach to reduce post-menopausal bone loss.
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DOI：
10.1126/science.1198469
发表时间：
2011-01-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Tanoue T;Shima T;Imaoka A;Kuwahara T;Momose Y;Cheng G;Yamasaki S;Saito T;Ohba Y;Taniguchi T;Takeda K;Hori S;Ivanov II;Umesaki Y;Itoh K;Honda K
通讯作者：
Honda K
影响因子：
20.3
作者：
Riggs, BL;Khosla, S;Melton, LJ
通讯作者：
Melton, LJ
影响因子：
4.8
作者：
Jadlowiec, J;Koch, H;Sfeir, C
通讯作者：
Sfeir, C
影响因子：
4.4
作者：
Oakley, Brian B.;Fiedler, Tina L.;Fredricks, David N.
通讯作者：
Fredricks, David N.
影响因子：
3.7
作者：
CHAO, A;LEE, SM
通讯作者：
LEE, SM