Microcystins Presence in Mussels (M. galloprovincialis) and Water of Two Productive Mediterranean's Lagoons (Sardinia, Italy).
Microcystins Presence in Mussels (M. galloprovincialis) and Water of Two Productive Mediterranean's Lagoons (Sardinia, Italy).
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DOI：
10.1155/2017/3769245
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发表时间：
2017
影响因子：
--
通讯作者：
Demontis MP
中科院分区：
文献类型：
--
作者：
Baralla E;Varoni MV;Sedda T;Pasciu V;Floris A;Demontis MP
微囊藻毒素（Microcystins，MCs）是一种严重危害动物和人类健康的肝毒素。它们之间毒性最大的类型是MC-LR，其存在已在世界各地的不同水库中进行了调查。在这项工作中，微囊藻毒素进行了监测，在春季和夏季的水和贻贝（贻贝galloprovincialis）的两个撒丁岛泻湖：Cabras和Calich泻湖。在酶联免疫吸附试验（ELISA）和随后的质谱检测之前，开发了固相萃取方法来清洁和浓缩样品。在两个泻湖中使用筛选ELISA试验检测MC的存在。Cabras泻湖的水和贻贝的MCs峰值出现在7月（水为0.75 ± 0.07纳克/升，贻贝为0.12 ± 0.04纳克/克湿重）。在Calich泻湖的水中，在所考虑的月份中，MCs的浓度呈恒定趋势，而贻贝中的MCs在7月达到峰值（0.6 ± 0.5 ng/g湿重）。以下LC-MS/MS分析未显示所有分析样品中均存在MC-LR。本研究结果对丰富公共卫生知识和消费者安全保障具有一定的参考价值。
Microcystins (MCs) are hepatotoxins harmful for animal and human health. The most toxic type between them is MC-LR whose presence has been investigated in different reservoirs all around the world. In this work microcystins were monitored in spring and summer in water and mussels (Mytilus galloprovincialis) of two Sardinia lagoons: Cabras and Calich lagoons. A Solid Phase Extraction method was developed to clean and concentrate samples before the Enzyme Linked Immunosorbent Assay (ELISA) and the following Mass Spectrometry detection. MCs presence was detected using the screening ELISA test in both lagoons. MCs peak was revealed in July for water and mussels belonging to Cabras lagoon (0.75 ± 0.07 ng/L in water and 0.12 ± 0.04 ng/g ww in mussels). In water of Calich lagoon there was a constant trend in the concentration of MCs during the considered months, while there was a MCs peak in July (0.6 ± 0.5 ng/g ww) in mussels. The following LC-MS/MS analysis did not reveal MC-LR presence in all analyzed samples. These results can be useful to enrich knowledge on public health and consumer's safeguard.
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影响因子：
2
作者：
Pulina, S.;Padedda, B. M.;Luglie, A.
通讯作者：
Luglie, A.
影响因子：
2.5
作者：
Souchu, P;Gasc, A;Deslous-Paoli, JM
通讯作者：
Deslous-Paoli, JM
影响因子：
11.4
作者：
Poste, Amanda E.;Hecky, Robert E.;Guildford, Stephanie J.
通讯作者：
Guildford, Stephanie J.
影响因子：
4.6
作者：
Mariani MA;Padedda BM;Kaštovský J;Buscarinu P;Sechi N;Virdis T;Lugliè A
通讯作者：
Lugliè A
影响因子：
5.4
作者：
Pearson L;Mihali T;Moffitt M;Kellmann R;Neilan B
通讯作者：
Neilan B