Hydrogen Sulfide Mediates K(+) and Na(+) Homeostasis in the Roots of Salt-Resistant and Salt-Sensitive Poplar Species Subjected to NaCl Stress.
Hydrogen Sulfide Mediates K(+) and Na(+) Homeostasis in the Roots of Salt-Resistant and Salt-Sensitive Poplar Species Subjected to NaCl Stress.
复制标题
硫化氢介导遭受 NaCl 胁迫的抗盐和盐敏感杨树根部 K 和 Na 稳态
DOI：
10.3389/fpls.2018.01366
复制
发表时间：
2018
影响因子：
5.6
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Zhao N;Zhu H;Zhang H;Sun J;Zhou J;Deng C;Zhang Y;Zhao R;Zhou X;Lu C;Lin S;Chen S
采用无创微测试技术（NMT）分析了nacl对两种杨树(耐盐杨树（Populus euphratica）和盐敏感杨树（popularis popularis）根系K+、Na+和H+通量的影响，以及NaHS （H2S供体）对根系离子通量的影响。两种杨树在盐胁迫（100 mM NaCl）、短期（24 h）和长期（LT） （5 d）盐胁迫（50 mM NaCl，称为盐胁迫）处理后均表现出净K+流出。NaHS （50 μM）抑制nacl诱导的根内K+外排，与盐暴露时间无关，但在低盐胁迫下胡杨的根内K+外排不明显。在低盐胁迫和NaHS处理下，两种杨根样品中，K+通道阻滞剂四乙基氯化铵（TEA）均能抑制nacl诱导的K+外排，但在质膜（PM） H+- atp酶特异性抑制剂正钒酸钠（sodium orthovanadate）的作用下，K+损失增加。这表明nacl诱导的K+损失是通过去极化激活的K+通道进行的。在短盐胁迫和低盐胁迫下，NaHS使杨树根系的Na+外排增加，H+内流相应增加。nahs增强的H+内流在短期盐胁迫下的胡杨样品中不显著。正钒酸钠和amiloride（一种Na+/H+反向转运抑制剂）均能有效抑制nahs增强的Na+外排，表明h2s增强的Na+外排是由于PM中Na+的活性排除。因此，我们得出结论，H2S的有益作用可能来自于Na+/H+反转运体系统（H+泵和Na+/H+反转运体）的向上调节，该系统促进了Na+与H+在PM上的交换，同时限制了由膜去极化激活的通道介导的K+损失。
Non-invasive micro-test techniques (NMT) were used to analyze NaCl-altered flux profiles of K+, Na+, and H+ in roots and effects of NaHS (a H2S donor) on root ion fluxes in two contrasting poplar species, Populus euphratica (salt-resistant) and Populus popularis (salt-sensitive). Both poplar species displayed a net K+ efflux after exposure to salt shock (100 mM NaCl), as well as after short-term (24 h), and long-term (LT) (5 days) saline treatment (50 mM NaCl, referred to as salt stress). NaHS (50 μM) restricted NaCl-induced K+ efflux in roots irrespective of the duration of salt exposure, but K+ efflux was not pronounced in data collected from the LT salt stress treatment of P. euphratica. The NaCl-induced K+ efflux was inhibited by a K+ channel blocker, tetraethylammonium chloride (TEA) in P. popularis root samples, but K+ loss increased with a specific inhibitor of plasma membrane (PM) H+-ATPase, sodium orthovanadate, in both poplar species under LT salt stress and NaHS treatment. This indicates that NaCl-induced K+ loss was through depolarization-activated K+ channels. NaHS caused increased Na+ efflux and a corresponding increase in H+ influx for poplar roots subjected to both the short- and LT salt stress. The NaHS-enhanced H+ influx was not significant in P. euphratica samples subjected to short term salt stress. Both sodium orthovanadate and amiloride (a Na+/H+ antiporter inhibitor) effectively inhibited the NaHS-augmented Na+ efflux, indicating that the H2S-enhanced Na+ efflux was due to active Na+ exclusion across the PM. We therefore conclude that the beneficial effects of H2S probably arise from upward regulation of the Na+/H+ antiport system (H+ pumps and Na+/H+ antiporters), which promote exchange of Na+ with H+ across the PM and simultaneously restricted the channel-mediated K+ loss that activated by membrane depolarization.
登录
查看更多内容
硫化氢通过一氧化氮介导维持大麦幼苗根部离子稳态来增强耐盐性
影响因子：
1.6
作者：
Cheng, Jingyi;Wan, Zhijun;Zhang, Peng
通讯作者：
Zhang, Peng
影响因子：
6.5
作者：
Lisjak, M.;Srivastava, N.;Hancock, J. T.
通讯作者：
Hancock, J. T.
影响因子：
6.9
作者：
Maathuis, FJM
通讯作者：
Maathuis, FJM
硫化氢和一氧化氮在减轻镉诱导的苜蓿幼苗根部氧化损伤中的作用
影响因子：
3.5
作者：
Li, Le;Wang, Yanqin;Shen, Wenbiao
通讯作者：
Shen, Wenbiao
影响因子：
5.2
作者：
Lai, Diwen;Mao, Yu;Xie, Yanjie
通讯作者：
Xie, Yanjie