Coordinated Regulation of Protoperithecium Development by MAP Kinases MAK-1 and MAK-2 in Neurospora crassa.
Coordinated Regulation of Protoperithecium Development by MAP Kinases MAK-1 and MAK-2 in Neurospora crassa.
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粗糙脉孢菌中 MAP 激酶 MAK-1 和 MAK-2 对原皮囊发育的协调调节。
DOI：
10.3389/fmicb.2021.769615
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发表时间：
2021
影响因子：
5.2
通讯作者：
Sun X
中科院分区：
文献类型：
--
作者：
Lan N;Ye S;Hu C;Chen Z;Huang J;Xue W;Li S;Sun X
有丝分裂原活化蛋白（MAP）激酶途径作为信号枢纽发挥作用，是许多重要细胞过程（包括性发育）不可或缺的。在无性发育过程中，PR和CWI MAP激酶通路之间的分子机制和相互作用已被广泛研究。然而，这些能否扩展到性发育仍然是一个谜。通过分析粗糙脉孢菌（Neurospora crassa）原囊形成过程中两个MAP激酶编码基因mak-2（PR-MAP kinase pathway）和mak-1（CWI-MAP kinase pathway）缺失后的全基因组转录响应，发现MAK-1和MAK-2蛋白共调控的430个基因在膜和氧化还原酶的组成部分功能丰富。这些基因包括13个参与性发育（app，poi-2，stk-17，fsd-1，vsd-8和NCU 03863）和黑色素合成（per-1，pkh-1，pkh-2，mld-1，scy-1，trn-2和trn-1）的功能已知基因，以及一组功能未知的基因。功能未知基因的缺失突变体的表型分析显示，12个基因是必要的女性生育。其中，NCU 07743（命名为pfd-1）、NCU 02250（oli）和NCU 05948（命名为pfd-2）的单基因缺失突变体表现出与Δmak-1和Δmak-2突变体相似的原囊发育缺陷，不能形成原囊。Western blotting分析表明，在Δnrc-1、Δmek-2和Δmak-2突变体中，磷酸化的MAK-1蛋白和总的MAK-1蛋白几乎被消除，表明MAK-1的转录后调控依赖于原围囊发育过程中的PR-MAP激酶通路。综上所述，本研究揭示了PR和CWI-MAP激酶途径在原围囊发育过程中的调控作用和相互作用。
Mitogen-activated protein (MAP) kinase pathways function as signaling hubs that are integral for many essential cellular processes, including sexual development. The molecular mechanisms and cross-talk between PR and CWI MAP kinase pathways have been extensively studied during asexual development. However, if these can be extended to sexual development remains elusive. By analyzing genome-wide transcriptional responses to deletion of each of two MAP kinase coding genes mak-2 (PR-MAP kinase pathway) and mak-1 (CWI-MAP kinase pathway) in Neurospora crassa during protoperithecium formation, 430 genes co-regulated by the MAK-1 and MAK-2 proteins were found, functionally enriched at integral components of membrane and oxidoreductase. These genes include 13 functionally known genes participating in sexual development (app, poi-2, stk-17, fsd-1, vsd-8, and NCU03863) and melanin synthesis (per-1, pkh-1, pkh-2, mld-1, scy-1, trn-2, and trn-1), as well as a set of functionally unknown genes. Phenotypic analysis of deletion mutants for the functionally unknown genes revealed that 12 genes were essential for female fertility. Among them, single-gene deletion mutants for NCU07743 (named as pfd-1), NCU02250 (oli), and NCU05948 (named as pfd-2) displayed similar protoperithecium development defects as the Δmak-1 and Δmak-2 mutants, failing to form protoperithecium. Western blotting analysis showed that both phosphorylated and total MAK-1 proteins were virtually abolished in the Δnrc-1, Δmek-2, and Δmak-2 mutants, suggesting that the posttranscriptional regulation of MAK-1 is dependent on the PR-MAP kinase pathway during the protoperithecium development. Taken together, this study revealed the regulatory roles and cross-talk between PR and CWI-MAP kinase pathways during protoperithecium development.
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作者：
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