Nanopore film based enrichment and quantification of low abundance hepcidin from human bodily fluids.
Nanopore film based enrichment and quantification of low abundance hepcidin from human bodily fluids.
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DOI：
10.1016/j.nano.2014.02.005
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发表时间：
2014-07
影响因子：
5.4
通讯作者：
Zhao, Yuliang
中科院分区：
文献类型：
--
作者：
Fan, Jia;Niu, Shiwen;Dong, Ailian;Shi, Jian;Wu, Hung-Jen;Fine, Daniel H.;Tian, Yaping;Zhou, Chunxi;Liu, Xuewu;Sun, Tong;Anderson, Gregory J.;Ferrari, Mauro;Nie, Guangjun;Hu, Ye;Zhao, Yuliang
内源性多肽代表了疾病的生物学和病理信息，引起了人们对诊断的兴趣。然而，由于人体体液(HBF)的复杂性，这些低丰度多肽的提取仍然是一个挑战。海普西丁是一种多肽激素，已被认为是铁相关疾病的生物标志物。没有快速可靠的方法来从HBF中浓缩它们。在这里，我们描述了一种基于纳米多孔二氧化硅薄膜的多肽提取方法，成功地从HBF中检测到了海普西丁。系统地研究了纳米孔对生物分子的协同作用，包括毛细管吸附、尺寸排斥和静电相互作用。为了将这一新方法推广到临床实践，我们进一步将其应用于成功地检测了健康志愿者和炎症患者提供的HBF中的海普西丁水平。我们的发现为从HBF中捕获和定量低丰度多肽提供了一种高通量、快速、无标记和低成本的检测方法。
Endogenous peptides that represent biological and pathological information of disease have attracted interest for diagnosis. However, the extraction of those low abundance peptides is still a challenge because of the complexity of human bodily fluids (HBF). Hepcidin, a peptide hormone, has been recognized as a biomarker for iron-related diseases. There is no rapid and reliable way to enrich them from HBF. Here we describe a peptides extraction approach based on nanoporous silica thin films to successfully detect hepcidin from HBF. Cooperative functions of nanopore to biomolecule, including capillary adsorption, size-exclusion and electrostatic interaction, were systematically investigated to immobilize the target peptide. To promote this new approach to clinical practices, we further applied it to successfully assay the hepcidin levels in HBF provided by healthy volunteers and patients suffering from inflammation. Our finding provides a high-throughput, rapid, label-free and cost-effective detection method for capturing and quantifying low abundance peptides from HBF.
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