Xeno-immunogenicity of ice-free cryopreserved porcine leaflets.
Xeno-immunogenicity of ice-free cryopreserved porcine leaflets.
复制标题
无冰冷冻保存的猪传单的异种免疫原性。
DOI：
10.1016/j.jss.2014.10.016
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Brockbank,KelvinGM
中科院分区：
文献类型：
--
作者：
Seifert,Martina;Bayrak,Alexandra;Stolk,Meaghan;Souidi,Naima;Schneider,Maria;Stock,UlrichA;Brockbank,KelvinGM
炎症、钙化或免疫调节反应的不良过程是患者心脏瓣膜长期存活的限制因素。本研究旨在探讨未脱细胞异种心脏瓣膜无冰低温保存(IFC)对人体适应性免疫反应的调节作用。方法将未经处理、常规低温保存的新鲜猪瓣和IFC瓣膜与人血单个核细胞体外培养5天，对其进行检测。没有使用其他组织治疗方案来改变可能的免疫反应。用基于流式细胞术的技术分析单独或附加低剂量第二刺激的基质对细胞增殖和细胞因子释放的诱导。用荧光标记的B4异凝素免疫组织化学方法检测α-Gal表位的表达。但是，当与抗CD3的第二次触发联合测试时，IFC瓣膜显示与新鲜或CFC组织相比，T细胞的增殖显著减少，特别是效应器记忆T细胞。此外，干扰素γ(干扰素γ)、肿瘤坏死因子α和白介素10的细胞因子水平降低，与CFC组相比有显著差异。结论异种组织IFC可能是预防获得性免疫系统应答的一种合适的治疗方法或处理步骤。
BackgroundUndesirable processes of inflammation, calcification, or immune-mediated reactions are limiting factors in long-term survival of heart valves in patients. In this study, we target the modulatory effects of ice-free cryopreservation (IFC) of xenogeneic heart valve leaflet matrices, without decellularization, on the adaptive human immune responsesin vitro.MethodsWe tested porcine leaflet matrices from fresh untreated, conventionally cryopreserved (CFC), and IFC pulmonary valves by culturing them with human blood mononuclear cells for 5 din vitro. No other tissue treatment protocols to modify possible immune responses were used. Matrices alone or in addition with a low-dose second stimulus were analyzed for induction of proliferation and cytokine release by flow cytometry-based techniques. Evaluation of the α-Gal epitope expression was performed by immunohistochemistry with fluorochrome-labeled B4 isolectin.ResultsNone of the tested leaflet treatment groups directly triggered the proliferation of immune cells. But when tested in combination with a second trigger by anti-CD3, IFC valves showed significantly reduced proliferation of T cells, especially effector memory T cells, in comparison with fresh or CFC tissue. Moreover, the cytokine levels for interferon-γ (IFNγ), tumor necrosis factor α, and interleukin-10 were reduced for the IFC-treated group being significantly different compared with the CFC group. However, no difference between treatment groups in the expression of the α-Gal antigen was observed.ConclusionsIFC of xenogeneic tissue might be an appropriate treatment method or processing step to prevent responses of the adaptive immune system.
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影响因子：
14
作者：
Boeer, Ulrike;Lohrenz, Andrea;Wilhelmi, Mathias
通讯作者：
Wilhelmi, Mathias
植入冷冻保存的同种异体心脏瓣膜后，对供体 HLA 产生广泛而强烈的体液免疫反应。
影响因子：
2.7
作者：
M. Welters;F. Oei;M. Witvliet;L. Vaessen;A. Cromme;A. Bogers;W. Weimar;F. Claas
通讯作者：
F. Claas
影响因子：
14
作者：
Daly, K. A.;Liu, S.;Badylak, S. F.
通讯作者：
Badylak, S. F.
影响因子：
2.7
作者：
Huber, Agnes J. T.;Brockbank, Kelvin G. M.;Stock, Ulrich A.
通讯作者：
Stock, Ulrich A.
冷冻同种移植物中的间质冰形成：体内组织退化和钙化的可能原因。
DOI：
--
发表时间：
2000
期刊：
The Journal of heart valve disease.
影响因子：
--
作者：
Brockbank,KG;Lightfoot,FG;Song,YC;Taylor,MJ
通讯作者：
Taylor,MJ