Electrochemical detection of DNA damage induced by clenbuterol at a reduced graphene oxide-Nafion modified glassy carbon electrode
Electrochemical detection of DNA damage induced by clenbuterol at a reduced graphene oxide-Nafion modified glassy carbon electrode
复制标题
还原氧化石墨烯-Nafion修饰玻碳电极电化学检测瘦肉精诱导的DNA损伤
DOI：
10.1039/c6ay03022j
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发表时间：
2017-02
影响因子：
3.1
通讯作者：
Kokot Serge
中科院分区：
文献类型：
--
作者：
Lin Xiaoyun;Ni Yongnian;Pei Xueying;Kokot Serge
研制了一种可直接检测克伦特罗(CLB)致DNA损伤的电化学生物传感器。用还原氧化石墨烯-Nafion和dsDNA修饰玻碳电极，制备了dsDNA/RGO-Nafion/GCE传感器。用电化学阻抗谱(EIS)和循环伏安法(CV)对修饰电极进行了表征，并对电极构建的各个阶段的结果进行了解释。结果表明，鸟嘌呤和腺嘌呤在电极(dsDNA/RGO-Nafion/GCE)上的电化学氧化峰电流明显高于未处理电极(dsDNA/GCE)。在此基础上，利用该传感器测量了传感器膜中完整dsDNA和损伤dsDNA之间鸟嘌呤和腺嘌呤的氧化峰电流的变化，成功地检测到了DNA损伤。鸟嘌呤和腺嘌呤与ClB浓度在5.0×10~(-7)~4.0×10~(-6)mol·L~(-1)范围内呈良好的线性关系，检出限为3.2×10~(-7)mol L~(-1)，为间接测定ClB提供了一种较好的方法。
An electrochemical biosensor capable of direct detection of DNA damage induced by clenbuterol (CLB) has been developed. A glassy carbon electrode (GCE) was modified using reduced graphene oxide-Nafion and dsDNA to produce a dsDNA/RGO-Nafion/GCE sensor. The modified electrode was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), and the results at each stage of the electrode construction were interpreted. They indicated that the electrochemical oxidation peak currents of guanine and adenine at the electrode (dsDNA/RGO-Nafion/GCE) significantly increased as compared with those at the untreated electrode (dsDNA/GCE). Based on this, the sensor was used to measure the change of the oxidation peak currents for guanine and adenine between the intact and damaged dsDNA in the sensor films, and the DNA damage was successfully detected. Furthermore, the plot of peak current decline for the guanine and adenine versus the concentration of CLB was linear in the range of 5.0 x 10(-7) to 4.0 x 10(-6) mol L-1, with a limit of detection (LOD) of 3.2 x 10(-7) mol L-1, which provided a good method to determine CLB indirectly.
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DOI：
10.1081/clt-100105153
发表时间：
2001-01
期刊：
Journal of Toxicology: Clinical Toxicology
影响因子：
--
作者：
T. Y. Chan
通讯作者：
T. Y. Chan
影响因子：
8.8
作者：
Ling Wang;Ran Yang;J. Chen;Jian-jun Li;Ling‐bo Qu;Peter de B. Harrington
通讯作者：
Ling Wang;Ran Yang;J. Chen;Jian-jun Li;Ling‐bo Qu;Peter de B. Harrington
影响因子：
12.6
作者：
Yi Liu;N. Hu
通讯作者：
Yi Liu;N. Hu
基于半胱胺修饰金纳米颗粒的瘦肉精快速比色传感器
影响因子：
9.5
作者：
Kang, Jingyan;Zhang, Yujie;Wu, Aiguo
通讯作者：
Wu, Aiguo
影响因子：
10.9
作者：
Solji Park;Soojin Kim;T. Anjong;Sung Eun Lee;Jinheung Kim
通讯作者：
Solji Park;Soojin Kim;T. Anjong;Sung Eun Lee;Jinheung Kim