Superhigh-sensitivity photothermal monitoring of individual cell response to antitumor drug.
Superhigh-sensitivity photothermal monitoring of individual cell response to antitumor drug.
复制标题
超高灵敏度光热监测单个细胞对抗肿瘤药物的反应。
DOI：
10.1117/1.2405349
复制
发表时间：
2006
影响因子：
3.5
通讯作者：
Chambers,TimothyC
中科院分区：
文献类型：
--
作者：
Zharov,VladimirP;Galitovskiy,Valentin;Lyle,ChristopherS;Chambers,TimothyC
We describe and explore the capability of a photothermal (PT) assay with modified schematics for highly sensitive detection of individual cell response to antitumor drug impactin vitro. Specifically, we used the nonlinear differential PT test to measure distinctive changes of specific PT parameters after exposure of KB3 carcinoma cells to the antitumor drug vinblastine in the broad concentration range of. Verification of the PT assay was performed by comparison with multidrug-resistant cells and comparison with conventional assays evaluating cell viability, cytochrome c release, apoptosis induction, and cell size. We demonstrate that this system is capable of detecting drug-induced signals at a concentration threshold sensitivity at least seven orders of magnitude better than existing assays. We anticipate that this technique may serve as a convenient and rapid analytical tool to evaluate the presence of intracellular drug, with applications in high throughput screening assays and for studying drug uptake and distribution in more complex biological or clinical samples.
登录
查看更多内容
尼古丁、抗肿瘤药物和辐射对细胞吸收结构影响的光热评估
DOI：
10.1117/12.532110
发表时间：
2004
期刊：
Arzneimittel-Forschung
影响因子：
--
作者：
V. Zharov;V. Galitovsky;P. Chowdhury;T. Chambers
通讯作者：
T. Chambers
热透镜原理图中探测光束的波动作为细胞代谢、细胞凋亡、坏死和激光影响的潜在指标
影响因子：
2.3
作者：
V. Zharov;Y. Menyaev;E. Shashkov;E. Galanzha;B. Khlebtsov;Andrei V. Scheludko;D. Zimnyakov;V. Tuchin
通讯作者：
V. Tuchin
影响因子：
2.3
作者：
Cree, IA;Kurbacher, CM;Bruckner, HW
通讯作者：
Bruckner, HW
利用激光光热细胞计数法研究光动力效应对微生物的影响
影响因子：
0.9
作者：
D O Lapotko;V P Zharov;T R Romanovskaya;G S Kuchinskii
通讯作者：
G S Kuchinskii
短激光脉冲对空间和光谱异质生物组织的瞬时局部加热的影响
DOI：
10.1007/bf02457180
发表时间：
1991
期刊：
Il Nuovo Cimento D
影响因子：
--
作者：
V. Letokhov
通讯作者：
V. Letokhov