Volatile emissions of scented Alstroemeria genotypes are dominated by terpenes, and a myrcene synthase gene is highly expressed in scented Alstroemeria flowers.
Volatile emissions of scented Alstroemeria genotypes are dominated by terpenes, and a myrcene synthase gene is highly expressed in scented Alstroemeria flowers.
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DOI：
10.1093/jxb/err456
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发表时间：
2012-04
影响因子：
6.9
通讯作者：
Rogers HJ
中科院分区：
文献类型：
--
作者：
Aros D;Gonzalez V;Allemann RK;Müller CT;Rosati C;Rogers HJ
Alstroemeria原产于南美洲，以其色彩缤纷的花被片而闻名，Alstroemeria杂种是一种重要的切花。然而，与许多商业切花一样，几乎所有的商业Alstroemeria杂种都没有香味。品种'甜劳拉'是很少有香味的商业Alstroemeria杂交种之一。这些切花的挥发性排放特征的表征揭示了三种主要的萜烯化合物：（E）-香紫苏烯，蛇麻烯（也称为α-香紫苏烯），一种罗勒烯样化合物，和几个次要峰，其中一个被鉴定为月桂烯。这一特征与其亲本A完全不同。香石竹属。挥发性物质的排放在开花期达到峰值，在两个香味基因型，一致的品种。'甜劳拉'与推定的萜烯合酶基因AlstroTPS的最大表达。该基因优先在两个品种的花组织中表达。“甜蜜的劳拉”和A.香石竹属。来自cv的AlstroTPS基因结构的表征。“甜劳拉”将其作为III类萜烯脱氢酶的成员，并且预测的567个氨基酸序列将其置于亚家族TPS-b中。保守序列R28（R）X8 W和D321 DXXD是推定的Mg 2+结合位点，并且在大肠杆菌中表达的AlstroTPS的体外测定显示编码的酶具有月桂烯合酶活性，这与AlstroTPS在Alstroemeria cv.“甜蜜的劳拉”花
Native to South America, Alstroemeria flowers are known for their colourful tepals, and Alstroemeria hybrids are an important cut flower. However, in common with many commercial cut flowers, virtually all the commercial Alstroemeria hybrids are not scented. The cultivar ‘Sweet Laura’ is one of very few scented commercial Alstroemeria hybrids. Characterization of the volatile emission profile of these cut flowers revealed three major terpene compounds: (E)-caryophyllene, humulene (also known as α-caryophyllene), an ocimene-like compound, and several minor peaks, one of which was identified as myrcene. The profile is completely different from that of the parental scented species A. caryophyllaea. Volatile emission peaked at anthesis in both scented genotypes, coincident in cv. ‘Sweet Laura’ with the maximal expression of a putative terpene synthase gene AlstroTPS. This gene was preferentially expressed in floral tissues of both cv. ‘Sweet Laura’ and A. caryophyllaea. Characterization of the AlstroTPS gene structure from cv. ‘Sweet Laura’ placed it as a member of the class III terpene synthases, and the predicted 567 amino acid sequence placed it into the subfamily TPS-b. The conserved sequences R28(R)X8W and D321DXXD are the putative Mg2+-binding sites, and in vitro assay of AlstroTPS expressed in Escherichia coli revealed that the encoded enzyme possesses myrcene synthase activity, consistent with a role for AlstroTPS in scent production in Alstroemeria cv. ‘Sweet Laura’ flowers.
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影响因子：
7.4
作者：
D'Auria, JC;Chen, F;Pichersky, E
通讯作者：
Pichersky, E
影响因子：
13.8
作者：
Breeze, E;Wagstaff, C;Buchanan-Wollaston, V
通讯作者：
Buchanan-Wollaston, V
影响因子：
2.7
作者：
Dempster, EL;Pryor, KV;Rogers, HJ
通讯作者：
Rogers, HJ
影响因子：
3
作者：
Aizen, MA;Basilio, A
通讯作者：
Basilio, A
影响因子：
7.4
作者：
Dudareva, N;Pichersky, E
通讯作者：
Pichersky, E