Effects of dexamethasone, ascorbic acid and β-glycerophosphate on the osteogenic differentiation of stem cells in vitro.
Effects of dexamethasone, ascorbic acid and β-glycerophosphate on the osteogenic differentiation of stem cells in vitro.
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DOI：
10.1186/scrt328
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发表时间：
2013
影响因子：
7.5
通讯作者：
Handschel J
中科院分区：
文献类型：
--
作者：
Langenbach F;Handschel J
多能干细胞成骨分化的标准程序是用地塞米松(Dex)、抗坏血酸(Asc)和β-甘油磷酸(β-Gly)的混合物处理融合的单层。这篇综述描述了这些物质对导致骨髓基质干细胞成骨分化的细胞内信号级联反应的影响。我们的结论是，地塞米松通过FHL2/β-连环蛋白介导的转录激活来诱导Runx2的表达，并通过上调TAZ和MKP1的表达来增强Runx2的活性。ASC导致I型胶原(COL1)分泌增加，进而导致COL1/α2β1整合素介导的细胞内信号增加。来自β-Gly的磷酸盐是羟基磷灰石中磷酸盐的来源，此外还影响细胞内的信号分子。在此背景下，我们特别关注营养不良和骨骼特异性矿化之间的差异。
The standard procedure for the osteogenic differentiation of multipotent stem cells is treatment of a confluent monolayer with a cocktail of dexamethasone (Dex), ascorbic acid (Asc) and β-glycerophosphate (β-Gly). This review describes the effects of these substances on intracellular signaling cascades that lead to osteogenic differentiation of bone marrow stroma-derived stem cells. We conclude that Dex induces Runx2 expression by FHL2/β-catenin-mediated transcriptional activation and that Dex enhances Runx2 activity by upregulation of TAZ and MKP1. Asc leads to the increased secretion of collagen type I (Col1), which in turn leads to increased Col1/α2β1 integrin-mediated intracellular signaling. The phosphate from β-Gly serves as a source for the phosphate in hydroxylapatite and in addition influences intracellular signaling molecules. In this context we give special attention to the differences between dystrophic and bone-specific mineralization.
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影响因子：
3.4
作者：
Langenbach, Fabian;Naujoks, Christian;Smeets, Ralf;Berr, Karin;Depprich, Rita;Kuebler, Norbert;Handschel, Joerg
通讯作者：
Handschel, Joerg
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作者：
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通讯作者：
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