Suo Quan Wan Protects Mouse From Early Diabetic Bladder Dysfunction by Mediating Motor Protein Myosin Va and Transporter Protein SLC17A9
Suo Quan Wan Protects Mouse From Early Diabetic Bladder Dysfunction by Mediating Motor Protein Myosin Va and Transporter Protein SLC17A9
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锁泉丸通过介导运动蛋白肌球蛋白 Va 和转运蛋白 SLC17A9 保护小鼠免受早期糖尿病膀胱功能障碍
DOI：
10.3389/fphar.2019.00552
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发表时间：
2019-05
影响因子：
5.6
通讯作者：
Cao Hongying
中科院分区：
文献类型：
--
作者：
Wang Jing;Lian Dawei;Yang Xufeng;Xu Yifei;Chen Fangjun;Lin Weijun;Wang Rui;Tang Liyao;Ren Wenkang;Fu Lijun;Huang Ping;Cao Hongying
Objective: To investigate the effects of Suo Quan Wan (SQW), a traditional Chinese.herbal formula, on the overactive bladder (OAB) of type 2 diabetes mellitus (T2DM).mouse models, particularly on its function of mediating the gene and protein expression.levels of myosin Va and SLC17A9..Materials and Methods: After 4 weeks high-fat diet (HFD) feeding, C57BL/6J mice.were injected with streptozotocin (100 mg/kg) for four times. After 3 weeks, the diabetic.mice were treated with SQW for another 3 weeks. Voided stain on paper assay, fasting.blood glucose (FBG) test, and oral glucose tolerance test (OGTT) were conducted..Urodynamic test, tension test [a,b-methylene ATP, electrical-field stimulation (EFS), KCl,.and carbachol] and histomorphometry were also performed. Western blot analysis and.qPCR assays were used to quantify the expression levels of myosin Va and SLC17A9..Results: The diabetic mice exhibited decreased weight but increased water intake,.urine production, FBG, and OGTT. No significant changes were observed after 3.weeks SQW treatment. Urodynamic test indicated that the non-voiding contraction.(NVC) frequency, maximum bladder capacity (MBC), residual volume (RV), and bladder.compliance (BC) were remarkably increased in the diabetic mice, whereas the.voided efficiency (VE) was decreased as a feature of overactivity. Compared with.the model mice, SQW treatment significantly improved urodynamic urination with.decreased NVC, MBC, RV, and BC, and increased VE. Histomorphometry results.showed that the bladder wall of the diabetic mice thickened, and SQW effectively.attenuated the pathological alterations. The contract responses of bladder strips.to all stimulators were higher in the DSM strips of diabetic mice, whereas SQW.treatment markedly decreased the contraction response for all stimuli. Moreover, the.protein and gene expression levels of myosin Va and SLC17A9 were up-regulated.in the bladders of diabetic mice, but SQW treatment restored such alterations..Conclusion: T2DM mice exhibited the early phase of diabetic bladder dysfunction.(DBD) characterized by OAB and bladder dysfunction. SQW can improve the.bladder storage and micturition of DBD mice by mediating the protein and gene.expression levels of myosin Va and SLC17A9 in the bladder, instead of improving the.blood glucose level.
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影响因子：
6.6
作者：
Daneshgari, Firouz;Liu, Guiming;Imrey, Peter B.
通讯作者：
Imrey, Peter B.
SQW对缺乏TRPV1小鼠的膀胱功能的影响。
影响因子：
--
作者：
Lai H;Yan Q;Cao H;Chen P;Xu Y;Jiang W;Wu Q;Huang P;Tan B
通讯作者：
Tan B
影响因子：
1.6
作者：
Lee YB;Ah Lee J;Soobin Jang;Lim Lee H
通讯作者：
Lim Lee H
影响因子：
2.7
作者：
Xu YF;Liang ZJ;Kuang ZJ;Chen JJ;Wu J;Lu XE;Jiang WW;Fan PL;Tang LY;Li YT;Huang P;Cao HY
通讯作者：
Cao HY
影响因子：
24
作者：
R. Skyrme-Jones;R. O’Brien;K. Berry;I. Meredith
通讯作者：
R. Skyrme-Jones;R. O’Brien;K. Berry;I. Meredith