Proteomic Analysis of Roots Response to Potassium Deficiency and the Effect of TaHAK1-4A on K(+) Uptake in Wheat.
Proteomic Analysis of Roots Response to Potassium Deficiency and the Effect of TaHAK1-4A on K(+) Uptake in Wheat.
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根部对缺钾反应的蛋白质组学分析以及 TaHAK1-4A 对小麦钾吸收的影响
DOI：
10.3390/ijms232113504
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发表时间：
2022-11-04
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
钾（K+）是植物生长和逆境反应所必需的。土壤钾离子含量不足会导致小麦品质和产量下降。因此，阐明小麦对低钾胁迫反应的分子机制至关重要。本研究采用串联质量标签（TMT）技术对耐钾小麦品种“KN9204”幼苗期根系中差异丰富蛋白（DAPs）含量进行了研究。在lk处理的根中共鉴定出104个dap。重点介绍了LK条件下与碳水化合物和能量代谢、运输、应激反应和防御以及翻译后修饰相关的DAPs。我们发现了一种高亲和力的钾转运蛋白（TaHAK1-4A），在LK处理后显著上调。此外，TaHAK1-4A主要在根中表达，编码蛋白定位在质膜中。酵母的互补实验表明，TaHAK1-4A介导极端LK条件下的K+摄取。TaHAK1-4A的过表达增加了LK条件下拟南芥的鲜重和根长，并改善了LK条件下生长不良的拟南芥athak5突变体幼苗的生长。此外，在LK胁迫下，TaHAK1-4A基因的沉默降低了小麦根系的长度、干重、K+浓度和K+流入量。因此，TaHAK1-4A对暴露于LK胁迫下的根系吸收K+具有重要作用。研究结果揭示了LK胁迫对小麦蛋白质代谢的影响。此外，我们还发现了一个候选基因，可能与提高钾离子利用效率的小麦品系相关。
Potassium (K+) is essential for plant growth and stress responses. A deficiency in soil K+ contents can result in decreased wheat quality and productivity. Thus, clarifying the molecular mechanism underlying wheat responses to low-K+ (LK) stress is critical. In this study, a tandem mass tag (TMT)-based quantitative proteomic analysis was performed to investigate the differentially abundant proteins (DAPs) in roots of the LK-tolerant wheat cultivar “KN9204” at the seedling stage after exposure to LK stress. A total of 104 DAPs were identified in the LK-treated roots. The DAPs related to carbohydrate and energy metabolism, transport, stress responses and defense, and post-translational modifications under LK conditions were highlighted. We identified a high-affinity potassium transporter (TaHAK1-4A) that was significantly up-regulated after the LK treatment. Additionally, TaHAK1-4A was mainly expressed in roots, and the encoded protein was localized in the plasma membrane. The complementation assay in yeast suggested that TaHAK1-4A mediates K+ uptake under extreme LK conditions. The overexpression of TaHAK1-4A increased the fresh weight and root length of Arabidopsis under LK conditions and improved the growth of Arabidopsis athak5 mutant seedlings, which grow poorly under LK conditions. Moreover, silencing of TaHAK1-4A in wheat roots treated with LK stress decreased the root length, dry weight, K+ concentration, and K+ influx. Accordingly, TaHAK1-4A is important for the uptake of K+ by roots exposed to LK stress. Our results reveal the protein metabolic changes in wheat induced by LK stress. Furthermore, we identified a candidate gene potentially relevant for developing wheat lines with increased K+ use efficiency.
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影响因子：
5.6
作者：
Bourgine B;Guihur A
通讯作者：
Guihur A
影响因子：
4.8
作者：
Bienert, Gerd P.;Moller, Anders L. B.;Jahn, Thomas P.
通讯作者：
Jahn, Thomas P.
影响因子：
4.4
作者：
Cai K;Zeng F;Wang J;Zhang G
通讯作者：
Zhang G
影响因子：
7.4
作者：
Bañuelos, MA;Garciadeblas, B;Rodríguez-Navarro, A
通讯作者：
Rodríguez-Navarro, A
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF