Quantitation of cellular dynamics in growing Arabidopsis roots with light sheet microscopy.
Quantitation of cellular dynamics in growing Arabidopsis roots with light sheet microscopy.
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DOI：
10.1371/journal.pone.0021303
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Leibler S
中科院分区：
文献类型：
--
作者：
Sena G;Frentz Z;Birnbaum KD;Leibler S
为了了解动态发育过程，必须频繁且长时间地对活体组织进行成像。在细胞分辨率下广泛研究根发育，以了解植物模式形成和维持的基本机制。不幸的是，确保连续的样本获取，同时保持生理条件并防止光损伤，对测量植物根等生长器官的细胞动力学构成了主要障碍。我们提出了一种系统，该系统将通过光片荧光显微镜进行的光学切片与水培培养相结合，使我们能够以细胞分辨率对垂直生长的拟南芥根每隔几分钟并连续几天进行成像。我们描述了新颖的自动化程序来跟踪根尖的生长、跟踪细胞核并识别细胞分裂。我们通过收集有关分裂和核动力学的数据来展示该系统的功能。
To understand dynamic developmental processes, living tissues have to be imaged frequently and for extended periods of time. Root development is extensively studied at cellular resolution to understand basic mechanisms underlying pattern formation and maintenance in plants. Unfortunately, ensuring continuous specimen access, while preserving physiological conditions and preventing photo-damage, poses major barriers to measurements of cellular dynamics in growing organs such as plant roots. We present a system that integrates optical sectioning through light sheet fluorescence microscopy with hydroponic culture that enables us to image, at cellular resolution, a vertically growing Arabidopsis root every few minutes and for several consecutive days. We describe novel automated routines to track the root tip as it grows, to track cellular nuclei and to identify cell divisions. We demonstrate the system's capabilities by collecting data on divisions and nuclear dynamics.
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影响因子：
64.8
作者：
Sena, Giovanni;Wang, Xiaoning;Liu, Hsiao-Yun;Hofhuis, Hugo;Birnbaum, Kenneth D.
通讯作者：
Birnbaum, Kenneth D.
影响因子：
20.6
作者：
Frigo, M;Johnson, SG
通讯作者：
Johnson, SG
影响因子：
3.8
作者：
Fuchs, E;Jaffe, JS;Azam, F
通讯作者：
Azam, F
影响因子：
7.2
作者：
Liu, Min;Yadav, Ram Kishor;Reddy, G. Venugopala
通讯作者：
Reddy, G. Venugopala
影响因子：
5.1
作者：
Baskin, TI
通讯作者：
Baskin, TI