Organ regeneration does not require a functional stem cell niche in plants.
Organ regeneration does not require a functional stem cell niche in plants.
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DOI：
10.1038/nature07597
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发表时间：
2009-02-26
期刊：
影响因子：
64.8
通讯作者：
Birnbaum, Kenneth D.
中科院分区：
文献类型：
--
作者：
Sena, Giovanni;Wang, Xiaoning;Liu, Hsiao-Yun;Hofhuis, Hugo;Birnbaum, Kenneth D.
植物依靠维持其顶端的干细胞生态位来维持根和芽的持续生长。然而，虽然植物细胞的发育可塑性已经被证明，但尚不清楚干细胞生态位是否是器官发生所必需的。在这里，我们探索广泛的分化细胞在没有干细胞利基活动的情况下再生器官的能力。使用拟南芥的根尖再生系统来跟踪细胞命运的分子和功能恢复，我们表明，丢失的细胞身份的重新指定在切除后几个小时内开始，特化细胞的功能在一天内恢复。关键的是，再生是在突变不能维持干细胞生态位的植物中进行的。这些结果表明，介导完整器官再生的干细胞样属性分散在植物分生组织中，并不局限于利基，但似乎对不确定的生长是必要的。这种对整个器官的再生重新编程不需要过渡到刻板的干细胞环境，这与最近关于诱导动物成年器官特定细胞类型的转分化的报道有有趣的相似之处。
Plants rely on the maintenance of stem cell niches at their apices for the continuous growth of roots and shoots. However, while the developmental plasticity of plant cells has been demonstrated, it is not known whether the stem cell niche is required for organogenesis. Here we explore the capacity of a broad range of differentiating cells to regenerate an organ without the activity of a stem cell niche. Using a root-tip regeneration system in Arabidopsis to track the molecular and functional recovery of cell fates, we show that re-specification of lost cell identities begins within hours of excision and that the function of specialized cells is restored within one day. Critically, regeneration proceeds in plants with mutations that fail to maintain the stem cell niche. These results show that stem cell-like properties that mediate complete organ regeneration are dispersed in plant meristems and are not restricted to niches, which nonetheless appear necessary for indeterminate growth. This regenerative reprogramming of an entire organ without transition to a stereotypical stem cell environment has intriguing parallels to recent reports of induced transdifferentiation of specific cell types in the adult organs of animals.
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影响因子：
64.5
作者：
Nemhauser, Jennifer L.;Hong, Fangxin;Chory, Joanne
通讯作者：
Chory, Joanne
DOI：
10.1073/pnas.091062498
发表时间：
2001-04-24
影响因子：
11.1
作者：
Tusher, VG;Tibshirani, R;Chu, G
通讯作者：
Chu, G
影响因子：
4.3
作者：
Sack, FD
通讯作者：
Sack, FD
影响因子：
56.9
作者：
Xu, J;Hofhuis, H;Scheres, B
通讯作者：
Scheres, B
影响因子：
11.6
作者：
Wang, JW;Wang, LJ;Chen, XY
通讯作者：
Chen, XY