N-glycosylation influences the latency and catalytic properties of mammalian purple acid phosphatase.
N-glycosylation influences the latency and catalytic properties of mammalian purple acid phosphatase.
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N-糖基化影响哺乳动物紫色酸性磷酸酶的潜伏期和催化特性。
DOI：
10.1016/j.abb.2004.11.029
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发表时间：
2005
影响因子：
3.9
通讯作者：
G. Andersson
中科院分区：
文献类型：
--
作者：
Yunling Wang;M. Norgård;G. Andersson
紫色酸性磷酸酶（PAP），也称为抗酒石酸酸性磷酸酶或子宫铁蛋白，含有两个潜在的共识N-糖基化位点，在Asn 97和Asn 128。在这项研究中，内源性大鼠骨PAP被发现具有类似的N-聚糖结构的大鼠重组PAP异源表达在杆状病毒感染的Sf 9昆虫细胞。来自Sf 9细胞的PAP显示含有两个N-连接的寡糖，而哺乳动物CHO-K1细胞表达的PAP糖基化程度较低。的N-糖基化的程度影响的酶的催化性能，作为N97 Q和N128 Q突变体，含有一个单一的寡糖链，表现出较低的底物亲和力和催化活性相比，那些完全糖基化的PAP在天然的，单体状态。底物亲和力和催化活性的差异被废除和部分恢复，分别通过蛋白水解裂解的环域，表明N-糖基化的程度影响的抑制性环域与催化重要残基的相互作用。
Purple acid phosphatase (PAP), also known as tartrate-resistant acid phosphatase or uteroferrin, contains two potential consensus N-glycosylation sites at Asn97and Asn128. In this study, endogenous rat bone PAP was found to possess similar N-glycan structures as rat recombinant PAP heterologously expressed in baculovirus-infected Sf9 insect cells. PAP from Sf9 cells was shown to contain two N-linked oligosaccharides, whereas PAP expressed by mammalian CHO-K1 cells was less extensively glycosylated. The extent of N-glycosylation affected the catalytic properties of the enzyme, as N97Q and N128Q mutants, containing a single oligosaccharide chain, exhibited a lower substrate affinity and catalytic activity compared to those of the fully glycosylated PAP in the native, monomeric state. The differences in substrate affinity and catalytic activity were abolished and partially restored, respectively, by proteolytic cleavage in the loop domain, indicating that the extent of N-glycosylation influences the interaction of the repressive loop domain with catalytically important residues.
猪子宫铁蛋白的碳水化合物结构以及高甘露糖链在促进胎儿肝脏网状内皮细胞摄取中的作用。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Saunders,PT;Renegar,RH;Raub,TJ;Baumbach,GA;Atkinson,PH;Bazer,FW;Roberts,RM
通讯作者：
Roberts,RM
影响因子：
2.9
作者：
J. Orlando;T. Zirino;B. Quirk;B. Averill
通讯作者：
J. Orlando;T. Zirino;B. Quirk;B. Averill
子宫铁蛋白具有 N-天冬酰胺连接的高甘露糖型寡糖，其中含有 6-磷酸甘露糖。
DOI：
10.1073/pnas.81.10.2985
发表时间：
1984
影响因子：
11.1
作者：
Baumbach,GA;Saunders,PT;Bazer,FW;Roberts,RM
通讯作者：
Roberts,RM