High-throughput discovery of synthetic surfaces that support proliferation of pluripotent cells.
High-throughput discovery of synthetic surfaces that support proliferation of pluripotent cells.
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DOI：
10.1021/ja906089g
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发表时间：
2010-02-03
影响因子：
15
通讯作者：
Kiessling, Laura L.
中科院分区：
文献类型：
--
作者：
Derda, Ratmir;Musah, Samira;Orner, Brendan P.;Klim, Joseph R.;Li, Lingyin;Kiessling, Laura L.
促进细胞生长或分化的合成材料推动了组织工程和再生医学领域的发展。大多数功能性生物材料都是基于一些源自细胞表面受体蛋白质配体的肽序列。由于很少有蛋白质拥有单独可以与细胞表面受体结合的短肽序列，因此可以用这种方法靶向的受体库是有限的。然而，可能需要结合不同类别受体的材料来引导细胞生长和分化。为了提供促进细胞生长的新材料，我们利用噬菌体展示来鉴定与多能细胞表面结合的新型肽。使用人胚胎癌细胞（EC）作为诱饵，分离出大约3×104个潜在的细胞结合噬菌体克隆。使用酶联免疫测定缩小了库的范围：测试了 370 个克隆，并鉴定了 7 个细胞结合肽。其中，6个序列具有EC细胞结合能力。具体来说，当在金上显示烷硫醇的自组装单层（SAM）时，它们会介导细胞粘附。相应的可溶性肽阻断了这种粘附，表明所鉴定的肽序列是特异性的。它们也很实用。展示噬菌体衍生肽的合成表面支持未分化的人类胚胎干 (ES) 细胞的生长。当这些细胞在化学成分确定的培养基 (mTeSR) 中呈现序列 TVKHRPDALHPQ 或 LTTAPKLPKVTR 的 SAM 上培养时，它们表达多能性标记物的水平与在 Matrigel 上培养的细胞相似。我们的结果表明，这种筛选策略是产生控制细胞生长和分化的材料的有效途径。
Synthetic materials that promote the growth or differentiation of cells have advanced the fields of tissue engineering and regenerative medicine. Most functional biomaterials are based on a handful of peptide sequences derived from protein ligands for cell surface receptors. Because few proteins possess short peptide sequences that alone can engage cell surface receptors, the repertoire of receptors that can be targeted with this approach is limited. Materials that bind diverse classes of receptors, however, may be needed to guide cell growth and differentiation. To provide access to new materials that promote cell growth, we utilized phage display to identify novel peptides that bind to the surface of pluripotent cells. Using human embryonal carcinoma (EC) cells as bait, approximately 3×104 potential cell-binding phage clones were isolated. The pool was narrowed using an enzyme-linked immunoassay: 370 clones were tested, and seven cell-binding peptides were identified. Of these, six sequences possess EC cell-binding ability. Specifically, when displayed by self-assembled monolayers (SAMs) of alkane thiols on gold they mediate cell adhesion. The corresponding soluble peptides block this adhesion, indicating that the identified peptide sequences are specific. They also are functional. Synthetic surfaces displaying phage-derived peptides support growth of undifferentiated human embryonic stem (ES) cells. When these cells were cultured on SAMs presenting the sequences TVKHRPDALHPQ or LTTAPKLPKVTR in chemically defined media (mTeSR), they express markers of pluripotency at levels similar to those of cells cultured on Matrigel. Our results indicate that this screening strategy is a productive avenue for the generation of materials that control the growth and differentiation of cells.
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影响因子：
82.9
作者：
Kolonin, MG;Saha, PK;Arap, W
通讯作者：
Arap, W
影响因子：
82.9
作者：
Arap, W;Kolonin, MG;Pasqualini, R
通讯作者：
Pasqualini, R
影响因子：
82.9
作者：
Giordano, RJ;Cardó-Vila, M;Arap, W
通讯作者：
Arap, W
影响因子：
15
作者：
Behanna, HA;Donners, JJJM;Stupp, SI
通讯作者：
Stupp, SI
DOI：
10.1016/j.bbrc.2008.07.111
发表时间：
2008-10-10
影响因子：
3.1
作者：
Miyazaki, Takamichi;Futaki, Sugiko;Suemori, Hirofumi
通讯作者：
Suemori, Hirofumi