Intra-operative imaging of surgical margins of canine soft tissue sarcoma using optical coherence tomography.
Intra-operative imaging of surgical margins of canine soft tissue sarcoma using optical coherence tomography.
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DOI：
10.1111/vco.12448
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发表时间：
2019-03
影响因子：
2.1
通讯作者：
Boppart SA
中科院分区：
文献类型：
--
作者：
Selmic LE;Samuelson J;Reagan JK;Mesa KJ;Driskell E;Li J;Marjanovic M;Boppart SA
光学相干断层扫描（OCT）是一种快速的非侵入性成像技术，在人类乳腺癌临床试验中显示出对术中手术切缘评估的高灵敏度。这项有前途的技术尚未在兽医学中进行评估。本研究的目的是将正常和异常的组织学特征与切除的犬软组织肉瘤（STS）手术切缘的OCT图像相关联，并建立识别阳性手术切缘的图像评价标准。对14只客户拥有的犬进行STS手术切除，并对切除标本上的2至4个感兴趣区域进行OCT成像。成像后，用外科墨水标记这些区域并修剪以进行组织病理学评价。结果表明，不同类型的组织在OCT图像上具有不同的特征性表现。脂肪组织表现出相对较低的散射和蜂窝状纹理图案。骨骼肌和肉瘤组织均致密，高度分散。虽然肉瘤组织是高度分散的，它没有组织的可识别的结构，相反，肌肉显示清晰的纤维排列模式。在这项研究中，我们发现不同的组织类型具有不同的和特征性的散射和图像纹理外观的OCT，这与低功率组织学图像密切相关。考虑到组织类型之间的差异，结果支持OCT可用于在STS切除后立即识别阳性手术切缘。需要进一步的研究来评估这种方法对手术切缘评估的诊断准确性。
Optical coherence tomography (OCT) is a rapid non-invasive imaging technique that has shown high sensitivity for intra-operative surgical margin assessment in human breast cancer clinical trials. This promising technology has not been evaluated in veterinary medicine. The objective of this study was to correlate normal and abnormal histological features with OCT images for surgical margins from excised canine soft tissue sarcoma (STS) and to establish image evaluation criteria for identifying positive surgical margins. Fourteen client-owned dogs underwent surgical resection of a STS and OCT imaging of 2 to 4 areas of interest on the resected specimen were performed. Following imaging these areas were marked with surgical ink and trimmed for histopathology evaluation. Results showed that different tissue types had distinct characteristic appearances on OCT imaging. Adipose tissue exhibited a relatively low scattering and a honey-comb texture pattern. Skeletal muscle and sarcoma tissue were both dense and highly scattering. While sarcoma tissue was highly scattering, it did not have organized recognizable structure in contrast to muscle which showed clear fibre alignment patterns. In this investigation, we showed different tissue types had different and characteristic scattering and image texture appearances on OCT, which closely correlate with low-power histology images. Given the differentiation between tissue types the results support that OCT could be used to identify positive surgical margins immediately following resection of STS. Further research is needed to assess the diagnostic accuracy of this method for surgical margin assessment.
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影响因子：
6.2
作者：
Keating JJ;Runge JJ;Singhal S;Nims S;Venegas O;Durham AC;Swain G;Nie S;Low PS;Holt DE
通讯作者：
Holt DE
影响因子：
1.8
作者：
DeWitt, Suzanne Bartholf;Eward, William C.;Berg, John
通讯作者：
Berg, John
影响因子：
2.4
作者：
McSporran, K. D.
通讯作者：
McSporran, K. D.
DOI：
10.2460/javma.240.10.1181
发表时间：
2012-05-15
影响因子：
1.9
作者：
Scarpa, Filippo;Sabattini, Silvia;Bettini, Giuliano
通讯作者：
Bettini, Giuliano
影响因子：
7.8
作者：
Gabon, Quentin;Sayag, David;Carozzo, Claude
通讯作者：
Carozzo, Claude