PRIMORDIAL GERM CELL‐DERIVED MOUSE EMBRYONIC GERM (EG) CELLSIN VITRORESEMBLE UNDIFFERENTIATED STEM CELLS WITH RESPECT TO DIFFERENTIATION CAPACITY AND CELL CYCLE DISTRIBUTION
PRIMORDIAL GERM CELL‐DERIVED MOUSE EMBRYONIC GERM (EG) CELLSIN VITRORESEMBLE UNDIFFERENTIATED STEM CELLS WITH RESPECT TO DIFFERENTIATION CAPACITY AND CELL CYCLE DISTRIBUTION
复制标题
原始生殖细胞衍生的小鼠胚胎胚芽 (EG) 细胞在分化能力和细胞周期分布方面与未分化干细胞相似
作者：
J. Rohwedel;U. Sehlmeyer;Jin Shan;A. Meister;A. Wobus
研究了小鼠原始生殖细胞来源的胚胎生殖细胞系EG-1的体外分化能力。通过作为胚胎样聚集体培养，EG-1细胞分化为心脏、骨骼肌和神经元细胞，伴随着组织特异性基因和蛋白质的表达，如RT-PCR分析和间接免疫荧光所示。与D3系的胚胎干（ES）细胞相比，分化成心肌细胞和肌肉细胞的效率相对较低，而自发神经元分化比D3细胞更有效。此外，在未分化的EG细胞和ES细胞中分析了作为分化状态参数的细胞周期时相的分布，并与P19系胚胎癌（EC）细胞和分化的上皮样EPI-7细胞获得的数据进行了比较。流式细胞仪分析显示EG、EC和ES细胞的细胞周期时相分布相似。相反，体细胞分化的EPI-7细胞显示出较长的G1期和较短的S期和G2/M期。总之，我们的结果表明，EG细胞在体外的分化状态和能力类似于全能ES细胞。
Embryonic germ (EG) cells of line EG‐1 derived from mouse primordial germ cells were investigated for theirin vitrodifferentiation capacity. By cultivation as embryo‐like aggregates EG‐1 cells differentiated into cardiac, skeletal muscle and neuronal cells accompanied by the expression of tissue‐specific genes and proteins as shown by RT‐PCR analysis and indirect immunofluorescence. In comparison to embryonic stem (ES) cells of line D3 the efficiency of differentiation into cardiac and muscle cells was comparatively low, whereas spontaneous neuronal differentiation was more efficient than in D3 cells. Furthermore, the distribution of cell cycle phases as a parameter for the differentiation state was analysed in undifferentiated EG cells and ES cells and compared to data obtained for embryonic carcinoma (EC) cells of line P19 and differentiated, epithelioid EPI‐7 cells. Flow cytometric analysis revealed similar cell cycle phase distributions in EG, EC and ES cells. In contrast, the somatic differentiated EPI‐7 cells showed a longer G1‐phase and shorter S‐ and G2/M‐phases. Together, our results demonstrate that the differentiation state and capacity of EG cellsin vitroresemble that of totipotent ES cells.
DOI：
10.1073/pnas.78.12.7634
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
MARTIN, GR
通讯作者：
MARTIN, GR