Histopathological changes of rat brain after direct injection of Hb-vesicles (artificial oxygen carriers) and neurological impact in an intracerebral hemorrhage model.
Histopathological changes of rat brain after direct injection of Hb-vesicles (artificial oxygen carriers) and neurological impact in an intracerebral hemorrhage model.
复制标题
直接注射血红蛋白囊泡（人工氧载体）后大鼠大脑的组织病理学变化以及脑出血模型中的神经系统影响。
DOI：
10.1002/jbm.a.32164
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
E. Tsuchida
中科院分区：
文献类型：
--
作者：
H. Sakai;M. Okamoto;E. Ikeda;H. Horinouchi;Koichi Kobayashi;E. Tsuchida
对于在输血期间用作人工氧载体，已经广泛研究了Hb囊泡（HbV，250 nm）的安全性和有效性。然而，其神经毒性仍然未知。我们使用大鼠脑出血模型探讨了HbV在脑中的潜在不良影响。用七氟烷麻醉雄性Wistar大鼠，并将其固定在立体定位架上。然后将HbV或同源RBC悬液（[Hb] = 8.6 g/dL，20 μ L）注入右侧尾状核。所有动物均存活，体重增加，并保持健康状态直至处死;手术后前几天除外。然而，两组均显示后肢收缩轻微无力、偶尔共济失调/步态和立毛。中性粒细胞在第1天在两组血肿周围组织中积聚，但在第3天已消失。第1天，血肿周围组织中的小HbV浸润显著，巨噬细胞中检测到吞噬的HbV。血红素氧合酶-1和含铁血黄素沉积在3天后出现，反映了HbV和RBC的降解。在HbV组中甚至在28天后仍可检测到HbV，但在RBC组中未检测到残留RBC。两组均显示星形胶质细胞增殖，称为神经胶质增生，3天后用于组织重建。该研究显示脑内注射HbV和RBC对照对行为功能和脑组织反应的不良反应之间没有显著差异。
For use as artificial oxygen carriers during transfusion, the safety and efficacy of Hb-vesicles (HbV, 250 nm), have been investigated extensively. Nevertheless, their neurotoxicity remains unknown. We explored potential adverse effects of HbV in the brain using a rat intracerebral hemorrhage model. Male Wistar rats were anesthetized with sevoflurane and fixed on a stereotaxic frame. Then HbV or homologous RBC suspension ([Hb] = 8.6 g/dL, 20 microL) was injected into the right caudate nucleus. All animals survived, gained weight, and maintained their well-being until the time of sacrifice; except during the first few days after surgery. However, both groups showed slight weakness in hind leg retraction, occasional ataxia/gait, and piloerection. Neutrophils accumulated at the onset of injury in perihematomal tissues in both groups at 1st day, but had disappeared by 3 days. Infiltration of small HbV in the perihematomal tissue was prominent at 1st day; phagocytized HbV were detected in macrophages. Hemeoxygenase-1 and hemosiderin deposition appeared after 3 days, reflecting the degradation of both HbV and RBC. The HbVs were detectable even after 28 days in the HbV group, but no residual RBCs were detected in the RBC group. Both groups showed proliferation of astrocytes, named gliosis, for tissue reconstruction after 3 days. This study revealed no notable differences in adverse effects between the intra-cerebral injection of HbV and the RBC control on behavioral functions and brain tissue responses.
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实验性脑出血：裂解红细胞对脑水肿和血脑屏障通透性的影响。
DOI：
10.1007/978-3-7091-6738-0_65
发表时间：
2002
期刊：
Acta neurochirurgica. Supplement
影响因子：
--
作者：
Bhasin,RR;Xi,G;Hua,Y;Keep,RF;Hoff,JT
通讯作者：
Hoff,JT
DOI：
10.1152/ajpheart.2000.279.3.h908
发表时间：
2000-09-01
影响因子：
4.8
作者：
Sakai, H;Hara, H;Intaglietta, M
通讯作者：
Intaglietta, M
影响因子：
4.1
作者：
Xi, GH;Keep, RF;Hoff, JT
通讯作者：
Hoff, JT
影响因子：
4.1
作者：
Huang, FP;Xi, GH;Hoff, JT
通讯作者：
Hoff, JT