Interfacial interaction between transmembrane ocular mucins and adhesive polymers and dendrimers analyzed by surface plasmon resonance.
Interfacial interaction between transmembrane ocular mucins and adhesive polymers and dendrimers analyzed by surface plasmon resonance.
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通过表面等离子体共振分析的跨膜眼粘蛋白与粘附聚合物和树枝状聚合物之间的界面相互作用。
DOI：
10.1007/s11095-012-0761-1
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发表时间：
2012-08
影响因子：
3.7
通讯作者：
Ponchel G
中科院分区：
文献类型：
--
作者：
Bravo-Osuna I;Noiray M;Briand E;Woodward AM;Argüeso P;Molina Martínez IT;Herrero-Vanrell R;Ponchel G
开发第一种基于生物传感器芯片技术的体外方法，用于探测眼部跨膜粘蛋白与黏附聚合物和树枝状大分子之间的界面相互作用现象。采用表面等离子体共振(SPR)技术。在芯片表面制备了跨膜眼粘蛋白表面，并用QCM-D(耗散石英晶体微天平)和XPS(X射线光电子能谱)进行了表征。被测试的黏附分子是：透明质酸(HA)、羧甲基纤维素(CMC)、羟丙甲基纤维素(HPMC)、壳聚糖(CH)和聚酰胺胺树枝状大分子(PAMAM)。对于HA、CMC和HPMC，在眼科临床使用的浓度下，CH与眼部跨膜粘蛋白的界面相互作用似乎是生物黏附的强制链式互扩散。有趣的是，PAMAM树枝状大分子与跨膜眼粘蛋白发生了永久性的界面相互作用，无论它们的表面化学基团是什么，这表明了这些多价体系协同作用的重要性。聚合物与眼膜相关粘蛋白的界面相互作用顺序为：CH(1%)>；G4PAMAM-NH_2(2%)=G4PAMAM-OH(2%)>；G3.5PAMAM-COOH(2%)≫CMC(0.5%)=HA(0.2%)=HPMC(0.3%)。该方法有助于在分子水平上识别粘蛋白-聚合物之间的化学相互作用。结果证实了壳聚糖和二乙醇胺作为聚合物的有效性，能够增加药物在眼表面的滞留时间，从而提高其生物利用度。
Development of the first in vitro method based on biosensor chip technology designed for probing the interfacial interaction phenomena between transmembrane ocular mucins and adhesive polymers and dendrimers intended for ophthalmic administration. The surface plasmon resonance (SPR) technique was used. A transmembrane ocular mucin surface was prepared on the chip surface and characterized by QCM-D (Quartz Crystal Microbalance with Dissipation) and XPS (X-ray photoelectron spectroscopy). The mucoadhesive molecules tested were: hyaluronic acid (HA), carboxymethyl cellulose (CMC), hydroxypropylmethyl cellulose (HPMC), chitosan (Ch) and polyamidoamine dendrimers (PAMAM). While Ch originated interfacial interaction with ocular transmembrane mucins, for HA, CMC and HPMC, chain interdiffusion seemed to be mandatory for bioadherence at the concentrations used in ophthalmic clinical practise. Interestingly, PAMAM dendrimers developed permanent interfacial interactions with transmembrane ocular mucins whatever their surface chemical groups, showing a relevant importance of co-operative effect of these multivalent systems. Polymers developed interfacial interactions with ocular membrane-associated mucins in the following order: Ch(1 %) > G4PAMAM-NH2(2 %) = G4PAMAM-OH(2 %) > G3.5PAMAM-COOH(2 %)≫ CMC(0.5 %) = HA(0.2 %) = HPMC(0.3 %). The method proposed is useful to discern between the mucin-polymer chemical interactions at molecular scale. Results reinforce the usefulness of chitosan and den-drimers as polymers able to increase the retention time of drugs on the ocular surface and hence their bioavailability.
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影响因子：
5.8
作者：
DURRANI, AM;FARR, SJ;KELLAWAY, IW
通讯作者：
KELLAWAY, IW
DOI：
10.1097/aci.0b013e32830e6b04
发表时间：
2008-10
影响因子：
2.8
作者：
Mantelli F;Argüeso P
通讯作者：
Argüeso P
影响因子：
6.4
作者：
Guzman-Aranguez, Ana;Argueeso, Pablo
通讯作者：
Argueeso, Pablo
影响因子：
4.3
作者：
Kesimer, Mehmet;Sheehan, John K.
通讯作者：
Sheehan, John K.
影响因子：
7.4
作者：
Komolov, KE;Senin, II;Koch, KW
通讯作者：
Koch, KW