Individual Mycobacterium tuberculosis universal stress protein homologues are dispensable in vitro.
Individual Mycobacterium tuberculosis universal stress protein homologues are dispensable in vitro.
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DOI：
10.1016/j.tube.2010.03.013
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发表时间：
2010-07
期刊：
影响因子：
--
通讯作者：
Williams HD
中科院分区：
文献类型：
--
作者：
Hingley-Wilson SM;Lougheed KE;Ferguson K;Leiva S;Williams HD
结核分枝杆菌有10种通用应激蛋白，其功能尚不清楚。然而，蛋白质组学和转录组学分析表明，许多usp基因在缺氧条件下和响应于一氧化氮和一氧化碳，以及在M.巨噬细胞系的结核感染。这些USP中的六个是DosR调节子的一部分，并且这沿着它们的表达模式和其他细菌物种中USP突变体的表型，表明在Mtb的持久性和/或细胞内存活中的潜在作用。产生编码USP Rv 1996、Rv 2005 c、Rv 2026 c和Rv 2028 c的单个USP基因的敲除突变体，并检查它们在缺氧和其它胁迫条件下的生长和存活。尽管大多数usp基因在缺氧条件下被高度诱导，但突变不影响Mtb在这些条件下的长期存活，或对一系列应激条件的响应，所述应激条件被选择来代表芽孢杆菌在感染期间经历的环境应激，也不影响小鼠和人源性巨噬细胞系感染期间经历的环境应激.这些USP在Mtb中功能冗余的可能性仍然存在。
Mycobacterium tuberculosis has 10 universal stress proteins, whose function is unknown. However, proteomic and transcriptomic analyses have shown that a number of usp genes are significantly upregulated under hypoxic conditions and in response to nitric oxide and carbon monoxide, as well as during M. tuberculosis infection of macrophage cell lines. Six of these USPs are part of the DosR regulon and this, along with their expression pattern and the phenotypes of usp mutants in other bacterial species, suggests a potential role in the persistence and/or intracellular survival of Mtb. Knock-out mutants of individual usp genes encoding the USPs Rv1996, Rv2005c, Rv2026c and Rv2028c were generated and their growth and survival under hypoxic and other stress conditions examined. Although the majority of usp genes are highly induced in hypoxic conditions, mutation did not affect the long term survival of Mtb under these conditions, or in response to a range of stress conditions chosen to represent the environmental onslaughts experienced by the bacillus during an infection, nor during infection of mouse and human – derived macrophage cell lines. The possibility remains that these USPs are functionally redundant in Mtb.
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影响因子：
14.9
作者：
Blokpoel MC;Murphy HN;O'Toole R;Wiles S;Runn ES;Stewart GR;Young DB;Robertson BD
通讯作者：
Robertson BD
影响因子：
64.5
作者：
Boshoff, HIM;Reed, MB;Mizrahi, V
通讯作者：
Mizrahi, V
影响因子：
3.2
作者：
Fisher, MA;Plikaytis, BB;Shinnick, TM
通讯作者：
Shinnick, TM
影响因子：
3.1
作者：
Downing, KJ;Mischenko, VV;Mizrahi, V
通讯作者：
Mizrahi, V
DOI：
10.1073/pnas.1631248100
发表时间：
2003-08-19
影响因子：
11.1
作者：
Dahl, JL;Kraus, CN;Barry, CE
通讯作者：
Barry, CE