Tyrosine kinase receptor Axl enhances entry of Zaire ebolavirus without direct interactions with the viral glycoprotein.
Tyrosine kinase receptor Axl enhances entry of Zaire ebolavirus without direct interactions with the viral glycoprotein.
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DOI：
10.1016/j.virol.2011.04.002
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发表时间：
2011-07-05
期刊：
影响因子：
3.7
通讯作者：
Maury W
中科院分区：
文献类型：
--
作者：
Brindley MA;Hunt CL;Kondratowicz AS;Bowman J;Sinn PL;McCray PB Jr;Quinn K;Weller ML;Chiorini JA;Maury W
In a bioinformatics-based screen for cellular genes that enhance Zaire ebolavirus (ZEBOV) transduction, AXL mRNA expression strongly correlated with ZEBOV infection. A series of cell lines and primary cells were identified that require Axl for optimal ZEBOV entry. Using one of these cell lines, we identified ZEBOV entry events that are Axl-dependent. Interactions between ZEBOV-GP and the Axl ectodomain were not detected in immunoprecipitations and reduction of surface expressed Axl by RNAi did not alter ZEBOV-GP binding, providing evidence that Axl does not serve as a receptor for the virus. However, RNAi knock down of Axl reduced ZEBOV pseudovirion internalization and α-Axl antisera inhibited pseudovirion fusion with cellular membranes. Consistent with the importance of Axl for ZEBOV transduction, Axl transiently co-localized on the surface of cells with ZEBOV virus particles and was internalized during virion transduction. In total, these findings indicate that endosomal uptake of filoviruses is facilitated by Axl.
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影响因子：
4.8
作者：
Demarchi, F;Verardo, R;Schneider, C
通讯作者：
Schneider, C
DOI：
10.1073/pnas.92.10.4477
发表时间：
1995-05-09
影响因子：
11.1
作者：
LAWSON, ND;STILLMAN, EA;ROSE, JK
通讯作者：
ROSE, JK
影响因子：
8
作者：
Goruppi, S;Ruaro, E;Schneider, C
通讯作者：
Schneider, C
影响因子：
3.7
作者：
Bhattacharyya, Suchita;Warfield, Kelly L.;Ruthel, Gordon;Bavari, Sina;Aman, M. Javad;Hope, Thomas J.
通讯作者：
Hope, Thomas J.
影响因子：
5.8
作者：
Baribaud, Frederic;Doms, Robert W;Pohlmann, Stefan
通讯作者：
Pohlmann, Stefan