Transcription profiling of a recently colonised pyrethroid resistant Anopheles gambiae strain from Ghana.
Transcription profiling of a recently colonised pyrethroid resistant Anopheles gambiae strain from Ghana.
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DOI：
10.1186/1471-2164-8-36
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发表时间：
2007-01-29
期刊：
影响因子：
4.4
通讯作者：
Ranson H
中科院分区：
文献类型：
--
作者：
Müller P;Donnelly MJ;Ranson H
蚊子对用于处理蚊帐的拟除虫菊酯杀虫剂的抗药性威胁着撒哈拉以南非洲疟疾控制的可持续性。虽然靶位点不敏感等位基因的影响正在被广泛讨论，但杀虫剂解毒的影响--尽管同样重要--仍然难以捉摸。成功开发疟疾干预和管理的新工具需要全面了解杀虫剂抗药性，包括代谢抗药性机制。虽然三个酶家族（细胞色素P450、谷胱甘肽S-转移酶和羧酸酯酶）与杀虫剂解毒作用广泛相关，但单个酶的作用在很大程度上尚不清楚。在这里，组成型表达模式的基因pusimetry参与赋予拟除虫菊酯的抗性进行了研究，在最近殖民的拟除虫菊酯抗性冈比亚按蚊菌株从Odumasy，加纳南部。提取来自两种性别的抗性菌株和标准实验室敏感菌株的RNA，逆转录并用Cy 3-或Cy 5-染料标记。标记的cDNA与detox芯片共杂交，detox芯片是一种定制的微阵列，含有超过230 A。gambiae基因片段，主要来自与杀虫剂抗性相关的酶家族。杂交后，测量Cy 3-和Cy 5-信号强度并逐基因比较。在耐药株的雌性和雄性中，细胞色素P450的CYP 6 Z 2和CYP 6 M2以及超氧化物歧化酶（SOD）基因家族的一个成员沿着高度过表达。这些基因不同于在东非拟除虫菊酯抗性A菌株中发现的上调基因。gambiae，并构成了一套新的候选基因涉及杀虫剂解毒。这些数据表明，代谢抗性可能有多个起源在A。冈比亚，这对抵抗管理具有重大影响。
Mosquito resistance to the pyrethroid insecticides used to treat bednets threatens the sustainability of malaria control in sub-Saharan Africa. While the impact of target site insensitivity alleles is being widely discussed the implications of insecticide detoxification – though equally important – remains elusive. The successful development of new tools for malaria intervention and management requires a comprehensive understanding of insecticide resistance, including metabolic resistance mechanisms. Although three enzyme families (cytochrome P450s, glutathione S-transferases and carboxylesterases) have been widely associated with insecticide detoxification the role of individual enzymes is largely unknown. Here, constitutive expression patterns of genes putatively involved in conferring pyrethroid resistance was investigated in a recently colonised pyrethroid resistant Anopheles gambiae strain from Odumasy, Southern Ghana. RNA from the resistant strain and a standard laboratory susceptible strain, of both sexes was extracted, reverse transcribed and labelled with either Cy3- or Cy5-dye. Labelled cDNA was co-hybridised to the detox chip, a custom-made microarray containing over 230 A. gambiae gene fragments predominantly from enzyme families associated with insecticide resistance. After hybridisation, Cy3- and Cy5-signal intensities were measured and compared gene by gene. In both females and males of the resistant strain the cytochrome P450s CYP6Z2 and CYP6M2 are highly over-expressed along with a member of the superoxide dismutase (SOD) gene family. These genes differ from those found up-regulated in East African strains of pyrethroid resistant A. gambiae and constitute a novel set of candidate genes implicated in insecticide detoxification. These data suggest that metabolic resistance may have multiple origins in A. gambiae, which has strong implications for the management of resistance.
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Anopheles Gambiae谷胱甘肽转移酶超值家族：注释，系统发育和表达谱。
影响因子：
4.4
作者：
Ding, YC;Ortelli, F;Rossiter, LC;Hemingway, J;Ranson, H
通讯作者：
Ranson, H
影响因子：
3.8
作者：
Kostaropoulos, I;Papadopoulos, AI;Papadopoulou-Mourkidou, E
通讯作者：
Papadopoulou-Mourkidou, E
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.1
作者：
Lehmann, T;Licht, M;Hawley, WA
通讯作者：
Hawley, WA
影响因子：
--
作者：
Awolola, TS;Brooke, BD;Coetzee, M
通讯作者：
Coetzee, M