The Anopheles gambiae glutathione transferase supergene family: annotation, phylogeny and expression profiles.
The Anopheles gambiae glutathione transferase supergene family: annotation, phylogeny and expression profiles.
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Anopheles Gambiae谷胱甘肽转移酶超值家族：注释，系统发育和表达谱。
DOI：
10.1186/1471-2164-4-35
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发表时间：
2003-08-13
期刊：
影响因子：
4.4
通讯作者：
Ranson, H
中科院分区：
文献类型：
--
作者：
Ding, YC;Ortelli, F;Rossiter, LC;Hemingway, J;Ranson, H
在冈比亚按蚊基因组中已鉴定出28个编码胞浆谷胱甘肽转移酶的pueridine基因。我们手动注释了这些基因，然后通过A.冈比亚cDNA。对果蝇的37个GST基因和其他类群的GST基因进行系统发育分析，以建立昆虫GST的命名法。昆虫GST的第二类先前已经涉及在几种昆虫物种中赋予杀虫剂抗性。我们比较了两株A.冈比亚，以确定是否EST-GST表达与杀虫剂抗性状态。两个A。冈比亚GST是选择性剪接的，产生最多32个编码胞质GST的转录物。我们在成年蚊子中检测了其中31种的cDNA。昆虫中至少有六种不同的GST，但A.冈比亚GST属于两种昆虫特有的纲，δ和γ。这两个GST类的成员分别聚集在染色体臂2L和3R上。GST超基因家族的两个成员是无内含子的。在其余的，有13个独特的内含子位置，但在内含子和δ类，有相当大的保守的内含子位置。八个GST基因中有五个在DDT抗性菌株中过表达。冈比亚。A.冈比亚是广泛的，这些基因的转录调节是复杂的。表达谱分析支持早期的预测，这一类是重要的，赋予杀虫剂的耐药性。
Twenty-eight genes putatively encoding cytosolic glutathione transferases have been identified in the Anopheles gambiae genome. We manually annotated these genes and then confirmed the annotation by sequencing of A. gambiae cDNAs. Phylogenetic analysis with the 37 putative GST genes from Drosophila and representative GSTs from other taxa was undertaken to develop a nomenclature for insect GSTs. The epsilon class of insect GSTs has previously been implicated in conferring insecticide resistance in several insect species. We compared the expression level of all members of this GST class in two strains of A. gambiae to determine whether epsilon GST expression is correlated with insecticide resistance status. Two A. gambiae GSTs are alternatively spliced resulting in a maximum number of 32 transcripts encoding cytosolic GSTs. We detected cDNAs for 31 of these in adult mosquitoes. There are at least six different classes of GSTs in insects but 20 of the A. gambiae GSTs belong to the two insect specific classes, delta and epsilon. Members of these two GST classes are clustered on chromosome arms 2L and 3R respectively. Two members of the GST supergene family are intronless. Amongst the remainder, there are 13 unique introns positions but within the epsilon and delta class, there is considerable conservation of intron positions. Five of the eight epsilon GSTs are overexpressed in a DDT resistant strain of A. gambiae. The GST supergene family in A. gambiae is extensive and regulation of transcription of these genes is complex. Expression profiling of the epsilon class supports earlier predictions that this class is important in conferring insecticide resistance.
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影响因子：
12.3
作者：
Dixon DP;Lapthorn A;Edwards R
通讯作者：
Edwards R
影响因子：
4.7
作者：
CLARK, AG;SHAMAAN, NA
通讯作者：
SHAMAAN, NA
影响因子：
64.8
作者：
GO, M
通讯作者：
GO, M
影响因子：
2.6
作者：
Ranson, H;Jensen, B;Collins, FH
通讯作者：
Collins, FH
DOI：
10.1073/pnas.95.24.14284
发表时间：
1998-11-24
影响因子：
11.1
作者：
Ranson, H;Collins, F;Hemingway, J
通讯作者：
Hemingway, J