Principal component analysis reveals age-related and muscle-type-related differences in protein carbonyl profiles of muscle mitochondria.
Principal component analysis reveals age-related and muscle-type-related differences in protein carbonyl profiles of muscle mitochondria.
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DOI：
10.1093/gerona/63.12.1277
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发表时间：
2008-12
期刊：
影响因子：
--
通讯作者：
Arriaga EA
中科院分区：
文献类型：
--
作者：
Feng J;Navratil M;Thompson LV;Arriaga EA
羰基修饰的蛋白质被认为是由氧化应激、衰老和疾病引起的氧化损伤的标志物。在这里，我们使用先前开发的毛细管电泳方法来检测线粒体蛋白质中的飞摩尔（10 - 15摩尔）羰基水平，这些蛋白质是大小分离的。对于蛋白质标记，用Alexa 488肼标记羰基，用3-（2-呋喃甲酰基）喹啉-2-甲醛标记蛋白质中的胺基。年轻Fischer 344大鼠的快收缩肌中总线粒体蛋白质羰基水平在统计学上高于慢收缩肌，老年大鼠的慢收缩肌中总线粒体蛋白质羰基水平在统计学上高于年轻大鼠。即使当总蛋白质羰基水平的一些统计比较不会显示差异时，主成分分析（PCA）将羰基谱分为四个不同年龄和肌肉类型的不同样本组。此外，PCA被用来预测，大多数年龄相关或肌肉类型相关的羰基水平的变化发生在蛋白质的分子量在9.8和11.7 kD之间。
Carbonyl-modified proteins are considered markers of oxidative damage caused by oxidative stress, aging, and disease. Here we use a previously developed capillary electrophoretic method for detecting femtomole (10−15 mole) carbonyl levels in mitochondrial proteins that are size separated and profiled. For protein labeling, carbonyls were tagged with Alexa 488 hydrazine and amine groups in proteins with 3-(2-furoyl)quinoline-2-carboxaldehyde. Total mitochondrial protein carbonyl levels were statistically higher in fast- than in slow-twitch muscle of young Fischer 344 rats, and statistically higher in old than in young slow-twitch muscle. Even when some statistical comparisons of the total protein carbonyl levels would not reveal differences, principal component analysis (PCA) classified the carbonyl profiles into four distinct sample groups of different age and muscle types. In addition, PCA was used to predict that most age-related or muscle-type-related changes in carbonyl levels occur in proteins with a molecular weight between 9.8 and 11.7 kD.
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影响因子：
3.9
作者：
Mosoni, L;Breuillé, D;Mirand, PP
通讯作者：
Mirand, PP
DOI：
10.1152/ajpendo.1996.271.6.e1044
发表时间：
1996-12-01
影响因子：
5.1
作者：
Madsen, K;Ertbjerg, P;Pedersen, PK
通讯作者：
Pedersen, PK
影响因子：
4.2
作者：
Bizzozero, OA;Ziegler, JL;Bolognani, F
通讯作者：
Bolognani, F
影响因子：
5.3
作者：
HOLLOSZY, JO;CHEN, M;YOUNG, JC
通讯作者：
YOUNG, JC
影响因子：
1.6
作者：
Bautista, J;Mateos-Nevado, MD
通讯作者：
Mateos-Nevado, MD