Kinetics of CD4+ T cell repopulation of lymphoid tissues after treatment of HIV-1 infection.
Kinetics of CD4+ T cell repopulation of lymphoid tissues after treatment of HIV-1 infection.
复制标题
HIV-1 感染治疗后淋巴组织 CD4 T 细胞再增殖的动力学。
DOI：
10.1073/pnas.95.3.1154
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发表时间：
1998
影响因子：
11.1
通讯作者：
A. T. Haase
中科院分区：
文献类型：
--
作者：
Zhi Qiang Zhang;D. Notermans;G. Sedgewick;W. Cavert;S. Wietgrefe;Mary L. Zupancic;K. Gebhard;K. Henry;Lawrence R. Boies;Zong;Marc K. Jenkins;R. Mills;H. McDade;C. Goodwin;C. Schuwirth;S. Danner;A. T. Haase
抗逆转录病毒药物的有效组合可减少有效感染 HIV-1 的 CD4+ T 淋巴细胞的周转，并减少淋巴组织 (LT) 中沉积的大量病毒粒子。为了确定 LT 中病毒复制的抑制和病毒抗原的减少可能在多大程度上相应地导致免疫系统的重新增殖，我们对 LT 中的 CD4+ T 淋巴细胞群体进行了表征，我们之前在治疗前后对病毒载量和受感染细胞的更新进行了定量。我们通过定量图像分析直接测量 CD4+ T 细胞总数、CD45RA+ 子集以及增殖或凋亡 CD4+ T 细胞分数的变化。与正常对照相比，我们发现 CD4+ T 细胞数量减少，增殖和凋亡增加。治疗后，增殖恢复到正常水平，CD4+ T 和 CD45RA+ 细胞总数增加。我们基于以下概念讨论了 HIV-1 对这一亚群的影响：成人的更新机制在感染前已满负荷运转，无法满足因有效感染细胞的损失而带来的额外需求。 CD45RA+ CD4+ T 细胞的缓慢增加与乐观的结论一致，即（i）即使在感染相对晚期阶段，更新机制也没有受到不可修复的损害，（ii）CD4+ T 细胞群可以通过控制主动复制而部分恢复，而无需根除 HIV-1。
Potent combinations of antiretroviral drugs diminish the turnover of CD4+ T lymphocytes productively infected with HIV-1 and reduce the large pool of virions deposited in lymphoid tissue (LT). To determine to what extent suppression of viral replication and reduction in viral antigens in LT might lead correspondingly to repopulation of the immune system, we characterized CD4+ T lymphocyte populations in LT in which we previously had quantitated viral load and turnover of infected cells before and after treatment. We directly measured by quantitative image analysis changes in total CD4+ T cell counts, the CD45RA+ subset, and fractions of proliferating or apoptotic CD4+ T cells. Compared with normal controls, we documented decreased numbers of CD4+ T cells and increased proliferation and apoptosis. After treatment, proliferation returned to normal levels, and total CD4+ T and CD45RA+ cells increased. We discuss the effects of HIV-1 on this subset based on the concept that renewal mechanisms in the adult are operating at full capacity before infection and cannot meet the additional demand imposed by the loss of productively infected cells. The slow increases in the CD45RA+ CD4+ T cells are consistent with the optimistic conclusions that (i) renewal mechanisms have not been damaged irreparably even at relatively advanced stages of infection and (ii) CD4+ T cell populations can be partially restored by control of active replication without eradication of HIV-1.
影响因子：
15.9
作者：
ROEDERER, M;DUBS, JG;HERZENBERG, LA
通讯作者：
HERZENBERG, LA
影响因子：
56.9
作者：
Cavert, W;Notermans, DW;Haase, AT
通讯作者：
Haase, AT