miR-153 inhibits the migration and the tube formation of endothelial cells by blocking the paracrine of angiopoietin 1 in breast cancer cells.
miR-153 inhibits the migration and the tube formation of endothelial cells by blocking the paracrine of angiopoietin 1 in breast cancer cells.
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miR-153通过阻断乳腺癌细胞中血管生成素1的旁分泌来抑制内皮细胞的迁移和管形成
DOI：
10.1007/s10456-018-9630-9
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发表时间：
2018-11
期刊：
影响因子：
9.8
通讯作者：
Chen C
中科院分区：
文献类型：
--
作者：
Liang H;Ge F;Xu Y;Xiao J;Zhou Z;Liu R;Chen C
内皮细胞的萌发是肿瘤血管生成的第一步。我们之前的研究表明miR-153部分通过靶向缺氧诱导因子（HIF1α）抑制乳腺肿瘤血管生成。在本研究中，我们证明了miR-153还通过直接靶向乳腺癌细胞中的血管生成素1 （ANG1）来抑制内皮细胞的迁移和成管。乳腺癌中miR-153与ANG1水平呈负相关。miR-153通过结合ANG1 mRNA阻断乳腺癌细胞中ANG1的表达和分泌。miR-153处理的乳腺癌细胞MCF7的条件培养基对HUVECs的增殖没有影响，但明显抑制HUVECs的迁移和成管，这可以通过过表达ANG1来挽救。此外，miR-153还通过下调ANG1直接抑制MCF7的增殖和迁移。这些发现表明，miR-153通过沉默ANG1抑制肿瘤细胞的活性以及内皮细胞的迁移和成管。
The sprouting of endothelial cells is the first step of tumor angiogenesis. Our previous study suggests that miR-153 suppresses breast tumor angiogenesis partially through targeting hypoxia-induced factor (HIF1α). In this study, we demonstrated that miR-153 also suppresses the migration and the tube formation of endothelial cells through directly targeting angiopoietin 1 (ANG1) in breast cancer cells. There was a negative correlation between miR-153 and ANG1 levels in breast cancer. miR-153 blocked the expression and secretion of ANG1 in breast cancer cells through binding to ANG1 mRNA. Conditioned medium from the breast cancer cell, MCF7, treated with miR-153 had no effect on the proliferation of HUVECs, but significantly inhibited the migration and tube formation of HUVECs, which could be rescued by overexpression of ANG1. In addition, miR-153 also directly inhibited the proliferation and migration of MCF7 through downregulation of ANG1. These findings suggest that miR-153 suppresses the activity of tumor cells and the migration and tube formation of endothelial cells by silencing ANG1.
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影响因子：
29.4
作者：
Taura, Kojiro;De Minicis, Saimuele;Brenner, David A.
通讯作者：
Brenner, David A.
缺氧通过 IRE1 α-XBP1 通路诱导 miR-153 微调乳腺癌血管生成中的 HIF1 α/VEGFA 轴
影响因子：
8
作者：
Liang H;Xiao J;Zhou Z;Wu J;Ge F;Li Z;Zhang H;Sun J;Li F;Liu R;Chen C
通讯作者：
Chen C
影响因子：
56.9
作者：
Maisonpierre, PC;Suri, C;Yancopoulos, GD
通讯作者：
Yancopoulos, GD
影响因子：
64.8
作者：
KIM, KJ;LI, B;FERRARA, N
通讯作者：
FERRARA, N
影响因子：
64.8
作者：
Risau, W
通讯作者：
Risau, W