Analysis of the light intensity dependence of the growth of Synechocystis and of the light distribution in a photobioreactor energized by 635 nm light.
Analysis of the light intensity dependence of the growth of Synechocystis and of the light distribution in a photobioreactor energized by 635 nm light.
复制标题
作者：
Cordara A;Re A;Pagliano C;Van Alphen P;Pirone R;Saracco G;Branco Dos Santos F;Hellingwerf K;Vasile N
集胞藻在建模研究和生物技术应用中的势头越来越大，这是由于多种因素，如快速生长，将二氧化碳固定到有价值的产品中的能力，以及相对容易的遗传操作。集胞藻的生理和代谢，因此，集胞藻为基础的光生物反应器（PBRs）的生产力，是严重的光调制。建立了集胞藻的实验室培养系统，研究了不同橙红光强度对集胞藻生长特性和光合活性的影响。集胞藻的生长和光合作用活性随着所提供的光强度增加到500 μmol光子m−2 s−1而增加，并且仅在800 μmol光子m−2 s−1时进入光抑制状态。有趣的是，将光恢复到非光抑制强度揭示了集胞藻能够迅速恢复。此外，我们的表征显示了生长速率和细胞大小的变化之间的明显相关性，扩展了以前在其他蓝藻中观察到的现象。此外，我们应用了一种建模方法来模拟通过改变入射光强度对其在PBR容器内的局部分布所产生的影响。我们的模型模拟表明，集胞藻的光合活性可以通过精细调节入射到PBR上的光的强度来增强，以防止细胞经历光诱导的应力，并诱导它们利用在容器中形成的不同局部光强度的区域。在后一种情况下，局部光强度的不均匀分布将允许集胞藻优化使用光。
Synechocystis gathered momentum in modelling studies and biotechnological applications owing to multiple factors like fast growth, ability to fix carbon dioxide into valuable products, and the relative ease of genetic manipulation. Synechocystis physiology and metabolism, and consequently, the productivity of Synechocystis-based photobioreactors (PBRs), are heavily light modulated. Here, we set up a turbidostat-controlled lab-scale cultivation system in order to study the influence of varying orange–red light intensities on Synechocystis growth characteristics and photosynthetic activity. Synechocystis growth and photosynthetic activity were found to raise as supplied light intensity increased up to 500 μmol photons m−2 s−1 and to enter the photoinhibition state only at 800 μmol photons m−2 s−1. Interestingly, reverting the light to a non-photo-inhibiting intensity unveiled Synechocystis to be able to promptly recover. Furthermore, our characterization displayed a clear correlation between variations in growth rate and cell size, extending a phenomenon previously observed in other cyanobacteria. Further, we applied a modelling approach to simulate the effects produced by varying the incident light intensity on its local distribution within the PBR vessel. Our model simulations suggested that the photosynthetic activity of Synechocystis could be enhanced by finely regulating the intensity of the light incident on the PBR in order to prevent cells from experiencing light-induced stress and induce their exploitation of areas of different local light intensity formed in the vessel. In the latter case, the heterogeneous distribution of the local light intensity would allow Synechocystis for an optimized usage of light.
登录
查看更多内容
影响因子：
16.6
作者：
Ferrezuelo, Francisco;Colomina, Neus;Aldea, Marti
通讯作者：
Aldea, Marti
影响因子：
2.9
作者：
Burrows, Elizabeth H.;Wong, Weng-Keen;Ely, Roger L.
通讯作者：
Ely, Roger L.
影响因子：
4.4
作者：
Anfelt, Josefine;Hallstrom, Bjorn;Hudson, Elton P.
通讯作者：
Hudson, Elton P.
DOI：
10.1016/j.algal.2014.06.004
发表时间：
2014-07-01
影响因子：
5.1
作者：
Dechatiwongse, Pongsathorn;Srisamai, Suna;Hellgardt, Klaus
通讯作者：
Hellgardt, Klaus
影响因子：
27.5
作者：
Fang, Longfa;Ge, Haitao;Wang, Yingchun
通讯作者：
Wang, Yingchun