Phage display of combinatorial peptide libraries: application to antiviral research.
Phage display of combinatorial peptide libraries: application to antiviral research.
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DOI：
10.3390/molecules16053499
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发表时间：
2011-04-26
期刊：
影响因子：
--
通讯作者：
Tordo N
中科院分区：
文献类型：
--
作者：
Castel G;Chtéoui M;Heyd B;Tordo N
鉴于由新出现的或地方性病毒引起的疾病数量不断增加，迫切需要原创策略：（1）鉴定对新病毒有活性的新药，（2）处理已选择对现有抗病毒分子具有抗性的病毒突变体。在这种情况下，抗病毒肽构成了疾病预防和治疗的一个有前途的领域。这些抑制肽的鉴定和开发需要组合文库的高通量筛选。噬菌体展示是一种从高度多样化的组合文库中选择对特定靶具有选择性亲和力的独特分子的强大技术。在过去的15年中，已经探索了这种技术用于抗病毒目的和用于药物发现中的候选抑制肽的分离。本文综述了噬菌体展示技术在抗病毒研究和药物发现中的应用，并讨论了结合该技术强大的筛选潜力和互补的合理方法来确定最佳靶点的优化策略。通过结合这些方法，应该可以最大限度地选择具有强抗病毒潜力的分子。
Given the growing number of diseases caused by emerging or endemic viruses, original strategies are urgently required: (1) for the identification of new drugs active against new viruses and (2) to deal with viral mutants in which resistance to existing antiviral molecules has been selected. In this context, antiviral peptides constitute a promising area for disease prevention and treatment. The identification and development of these inhibitory peptides require the high-throughput screening of combinatorial libraries. Phage-display is a powerful technique for selecting unique molecules with selective affinity for a specific target from highly diverse combinatorial libraries. In the last 15 years, the use of this technique for antiviral purposes and for the isolation of candidate inhibitory peptides in drug discovery has been explored. We present here a review of the use of phage display in antiviral research and drug discovery, with a discussion of optimized strategies combining the strong screening potential of this technique with complementary rational approaches for identification of the best target. By combining such approaches, it should be possible to maximize the selection of molecules with strong antiviral potential.
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