A maternal-zygotic effect gene, Zfp57, maintains both maternal and paternal imprints.
A maternal-zygotic effect gene, Zfp57, maintains both maternal and paternal imprints.
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DOI：
10.1016/j.devcel.2008.08.014
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发表时间：
2008-10
影响因子：
11.8
通讯作者：
Ferguson-Smith, Anne C.
中科院分区：
文献类型：
--
作者：
Li, Xiajun;Ito, Mitsuteru;Zhou, Fen;Youngson, Neil;Zuo, Xiaopan;Leder, Philip;Ferguson-Smith, Anne C.
负责维持小鼠胚胎中基因组甲基化印记的机制尚不清楚。我们在编码KRAB锌指蛋白的Zfp57基因座中产生敲除小鼠。Zfp57合子功能的缺失导致部分新生儿致死，而消除Zfp57的母体和合子功能导致高度外显的胚胎致死。在卵母细胞中，Zfp57的缺失导致在Snrpn印迹区域不能建立母体甲基化印迹。有趣的是，当合子Zfp57存在于胚胎中时，甲基化印记特异性地在母体来源的Snrpn印记区域重新获得。这表明基因组印记可能存在DNA甲基化无关的记忆。Zfp57也是受精后维持多个印记结构域的母本和父本甲基化印记所必需的。对基因组印记的影响与Zfp57突变体的母合子致死性一致。
The mechanisms responsible for maintaining genomic methylation imprints in mouse embryos are not understood. We generated a knockout mouse in the Zfp57 locus encoding a KRAB zinc finger protein. Loss of just the zygotic function of Zfp57 causes partial neonatal lethality, while eliminating both maternal and zygotic functions of Zfp57 results in a highly penetrant embryonic lethality. In oocytes, absence of Zfp57 results in failure to establish maternal methylation imprints at the Snrpn imprinted region. Intriguingly, methylation imprints are re-acquired specifically at the maternally derived Snrpn imprinted region when the zygotic Zfp57 is present in embryos. This suggests that there may be DNA methylation-independent memory for genomic imprints. Zfp57 is also required for the post-fertilization maintenance of maternal and paternal methylation imprints at multiple imprinted domains. The effects on genomic imprinting are consistent with the maternal-zygotic lethality of Zfp57 mutants.
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DOI：
10.1073/pnas.041616998
发表时间：
2001-02-13
影响因子：
11.1
作者：
Abrink, M;Ortiz, JA;Losson, R
通讯作者：
Losson, R
影响因子：
10.5
作者：
Friedman, JR;Fredericks, WJ;Rauscher, FJ
通讯作者：
Rauscher, FJ
影响因子：
3.5
作者：
Kantor, B;Makedonski, K;Razin, A
通讯作者：
Razin, A
影响因子：
56.9
作者：
Humpherys, D;Eggan, K;Jaenisch, R
通讯作者：
Jaenisch, R
影响因子：
1.7
作者：
Kobayashi, H.;Suda, C.;Sasaki, H.
通讯作者：
Sasaki, H.