Cyclin mRNA and protein expression in recombinant interleukin 2-stimulated cloned murine T lymphocytes.
Cyclin mRNA and protein expression in recombinant interleukin 2-stimulated cloned murine T lymphocytes.
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重组白细胞介素 2 刺激的克隆鼠 T 淋巴细胞中细胞周期蛋白 mRNA 和蛋白的表达。
DOI：
10.1002/jcb.240380306
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发表时间：
1988
影响因子：
4
通讯作者：
Prystowsky,MB
中科院分区：
文献类型：
--
作者：
Shipman,PM;Sabath,DE;Fischer,AH;Comber,PG;Sullivan,K;Tan,EM;Prystowsky,MB
研究了在重组白介素2(RIL2)诱导的克隆T淋巴细胞细胞周期进程中，非组蛋白核蛋白Cyclin的表达。我们发现，免疫荧光检测到细胞周期蛋白的表达与rIL-2刺激的L2细胞的增殖密切相关，而不仅仅是与S期有关。流式细胞仪检测细胞周期各时相(G1/S/G2/M期)均可检测到细胞周期蛋白的免疫荧光。Northern印迹分析显示，细胞周期蛋白基因的表达在刺激后1h即可表达，在刺激后25~49h达到高峰，并在整个刺激过程中保持较高水平。从IL-2刺激的克隆T细胞的cDNA文库中克隆了小鼠细胞周期蛋白基因的编码区。测定了小鼠细胞周期蛋白基因5‘端的序列，发现其与大鼠和人细胞周期蛋白基因克隆的序列分别有88%和82%的相似性。目前的研究表明，在IL-2诱导的克隆T细胞增殖过程中，细胞周期蛋白和mRNA的积累受到高度调控。这些数据为研究细胞增殖过程中细胞周期蛋白基因表达调控的分子机制提供了一个框架。
Expression of cyclin, a non‐histone nuclear protein, during recombinant interleukin 2 (rIL2)‐driven cell‐cycle progression of cloned T lymphocytes has been assessed. We found that expression of cyclin protein, as detected by immunofluorescence, is tightly associated with proliferation, and not merely S‐phase, of L2 cells stimulated with rIL2. Cyclin immunofluorescence was detected in all cell‐cycle phases (G1/S/G2/M, as detected by flow cytometry) of proliferating L2 cells. Accumulation of cyclin mRNA levels was induced as early as 1 h after stimulation, was maximal at 25–49 h, and remained elevated throughout stimulation, as detected by Northern blot analysis. A cDNA‐encoding murine cyclin was cloned from a cDNA library prepared from IL2‐stimulated cloned T cells. The sequence of the 5′ end of the murine cyclin cDNA was determined and found to be 88% and 82% similar to the sequences of cDNA clones encoding rat and human cyclin, respectively. The present studies demonstrate that cyclin protein and mRNA accumulation are highly regulated during IL2‐induced proliferation of a cloned T cell. These data provide a framework for addressing the molecular mechanisms regulating cyclin gene expression during cellular proliferation.
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影响因子：
4.4
作者：
C. Tannenbaum;Nancy L. Wicker;D. Armstrong;R. Tubbs;J. Finke;R. Bukowski;T. Hamilton
通讯作者：
C. Tannenbaum;Nancy L. Wicker;D. Armstrong;R. Tubbs;J. Finke;R. Bukowski;T. Hamilton
DOI：
10.1073/pnas.87.14.5238
发表时间：
1990-07-01
影响因子：
11.1
作者：
FARBER, JM
通讯作者：
FARBER, JM
IL-12 通过诱导 IFN-γ 来防止感染荚膜组织胞浆菌的小鼠死亡。
影响因子：
4.4
作者：
Ping Zhou;M. Sieve;J. Bennett;K. J. Kwon;R. Tewari;R. Gazzinelli;Alan Sher;R. Seder
通讯作者：
R. Seder
DOI：
10.1084/jem.179.5.1551
发表时间：
1994-05-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Wynn TA;Eltoum I;Oswald IP;Cheever AW;Sher A
通讯作者：
Sher A
肾细胞癌患者肿瘤浸润淋巴细胞和外周血淋巴细胞Fas(APO-1/CD95)的表达
影响因子：
11.2
作者：
Cardi,G;Heaney,JA;Schned,AR;Ernstoff,MS
通讯作者：
Ernstoff,MS