Comparative study on methods of DNA genotyping between single piece of dandruff and EZ-tape
Comparative study on methods of DNA genotyping between single piece of dandruff and EZ-tape
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单块头皮屑与EZ-tape DNA基因分型方法的比较研究
DOI：
10.1016/j.fsigss.2017.09.097
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发表时间：
2017-12
影响因子：
--
通讯作者：
Weibo Liang
中科院分区：
文献类型：
--
作者：
Hui Jian;Jing Zhu;Hui Wang;Tao Feng;Peng Bai;Weibo Liang
头皮屑通常出现在刑事案件中，它含有一些有核细胞，使提取DNA用于法医用途成为可能。公安部法医学研究所研制的脱落细胞提取工具EZ-TAPE在中国中得到了广泛的应用。用EZ-TAPE收集头皮屑和其他不确定的颗粒，直接从EZ-TAPE中提取DNA。在这项研究中，我们旨在通过从3M苏格兰胶带中分离出单个头皮屑来进行后续DNA图谱分析，从而提高样本的利用率。我们比较了头皮屑和EZ胶带的DNA图谱结果。此外，我们还试图探索振荡是否会影响等位基因的恢复。结果表明，有振荡的单片头皮屑中有56%获得了完整的STR图谱，而没有振荡的单片头皮屑中只有11%获得了完整的STR图谱。单片头皮屑可以提供足够的DNA用于STR基因分型，混合样本很少(12.5%)能被鉴定出来。此外，两个贡献者都被检测到了。然而，通过EZ-TAPE收集的样本中有41.7%遗漏了一个贡献者，33.3%是混合体。因此，在一些特殊的法医案件中，处理单一的头皮屑可能是一种潜在的个人识别选择。
Dandruff is generally presented in criminal cases, and it contains some nucleated cells which make it possible to extract DNA for forensic uses. EZ-tape, an extraction tool for exfoliative cells, developed by Institute of Forensic Science, Ministry of Public Security, is widely used in China. Dandruff was collected by EZ-tape together with other indefinite particles, and DNA was extracted directly from EZ-tapes. In this study, we aimed to improve the utilization of samples by isolating single pieces of dandruff from 3 M scotch tapes for subsequent DNA profiling. We compared the DNA profile results of single pieces of dandruff and EZ-tape. In addition, we tried to explore whether the oscillation can affect the alleles recover. Results indicated that 56% of single pieces of dandruff with oscillation obtained full STR profiles compared with 11% with non-oscillation. Single pieces of dandruff could provide enough DNA for STR genotyping and rare sample (12.5%) could be identified mixture. Besides, both contributors are detected. However, 41.7% of samples collected through EZ-tape missed one contributor and 33.3% were mixture. Therefore, dealing with single piece of dandruff may be a potential alternative for individual identification in some special forensic casework.
影响因子：
3.1
作者：
Forsberg, Christina;Jansson, Linda;Hedman, Johannes
通讯作者：
Hedman, Johannes
DOI：
10.1016/j.fsigen.2016.11.004
发表时间：
2017-03
期刊：
Forensic science international. Genetics
影响因子：
--
作者：
I. Zupanič Pajnič;T. Zupanc;J. Balažič;Živa Miriam Geršak;O. Stojković;Ivan Skadrić;Matija Črešnar
通讯作者：
I. Zupanič Pajnič;T. Zupanc;J. Balažič;Živa Miriam Geršak;O. Stojković;Ivan Skadrić;Matija Črešnar
影响因子：
1.6
作者：
M. Lorente;C. Entrala;J. Lorente;J. C. Alvarez;E. Villanueva;B. Budowle
通讯作者：
M. Lorente;C. Entrala;J. Lorente;J. C. Alvarez;E. Villanueva;B. Budowle