Host genomic influence on bacterial composition in the switchgrass rhizosphere.
Host genomic influence on bacterial composition in the switchgrass rhizosphere.
复制标题
柳枝稷根际细菌组成对宿主基因组的影响。
DOI：
10.1111/mec.16549
复制
发表时间：
2022-07
影响因子：
4.9
通讯作者：
Lasky, Jesse R.
中科院分区：
文献类型：
--
作者：
Sutherland, Jeremy;Bell, Terrence;Trexler, Ryan V.;Carlson, John E.;Lasky, Jesse R.
寄主遗传变异可以塑造相关微生物群的多样性和组成，这可能会相互影响寄主的特征和表现。虽然自然界植物种群表型多样性的遗传基础已经被研究，但相对较少的研究调查了寄主对其相关微生物群的遗传学影响。柳枝菊是一种高度异交的多年生草本植物，在其原生北美范围内具有相当大的局部适应性多样性。在这里，我们比较了383份普通菜园的柳枝条材料，以确定寄主基因对根际细菌组成的影响。我们假设根际细菌组合的组成和多样性将由于寄主之间的基因差异而不同(可能是由于根的表型和相关的生活史变化)。我们观察到与旱地生态型和四倍体相关的细菌的α多样性分别高于低地生态型和八倍体。α多样性与开花时间和株高呈显著负相关，表明柳枝条的细菌组成在生活史轴线上存在差异。观察了21个核心细菌家族相对丰度的狭义遗传力(H2)。由于遗传变异，四倍体之间的总体组成差异支持对根际微生物群产生广泛的基因影响。四倍体只是因为与八倍体基因组相关的复杂性而被考虑。最后，一项全基因组关联研究确定了与110个家族相关的1861个单核苷酸多态以及包含它们的与潜在调控功能相关的基因。我们的发现表明，柳枝草基因组和生活史的变化影响了根际细菌的组成，可能是由于宿主对当地环境的适应。
Host genetic variation can shape the diversity and composition of associated microbiomes, which may reciprocally influence host traits and performance. While the genetic basis of phenotypic diversity of plant populations in nature has been studied, comparatively little research has investigated the genetics of host effects on their associated microbiomes. Switchgrass (Panicum virgatum) is a highly outcrossing, perennial, grass species with substantial locally adaptive diversity across its native North American range. Here, we compared 383 switchgrass accessions in a common garden to determine the host genotypic influence on rhizosphere bacterial composition. We hypothesized that the composition and diversity of rhizosphere bacterial assemblages would differentiate due to genotypic differences between hosts (potentially due to root phenotypes and associated life history variation). We observed higher alpha diversity of bacteria associated with upland ecotypes and tetraploids, compared to lowland ecotypes and octoploids, respectively. Alpha diversity correlated negatively with flowering time and plant height, indicating that bacterial composition varies along switchgrass life history axes. Narrow-sense heritability (h2) of the relative abundance of 21 core bacterial families was observed. Overall compositional differences among tetraploids, due to genetic variation, supports widespread genotypic influence on the rhizosphere microbiome. Tetraploids were only considered due to complexities associated with the octoploid genomes. Lastly, a genome-wide association study identified 1861 single-nucleotide polymorphisms associated with 110 families and genes containing them related to potential regulatory functions. Our findings suggest that switchgrass genomic and life-history variation influences bacterial composition in the rhizosphere, potentially due to host adaptation to local environments.
登录
查看更多内容
DOI：
10.1038/s41396-021-00993-z
发表时间：
2021-11
期刊：
The ISME journal
影响因子：
--
作者：
Deng S;Caddell DF;Xu G;Dahlen L;Washington L;Yang J;Coleman-Derr D
通讯作者：
Coleman-Derr D
影响因子：
4.5
作者：
Brown, Brielin C.;Bray, Nicolas L.;Pachter, Lior
通讯作者：
Pachter, Lior
将植物营养状况与植物-微生物相互作用联系起来
影响因子：
3.7
作者：
Carvalhais LC;Dennis PG;Fan B;Fedoseyenko D;Kierul K;Becker A;von Wiren N;Borriss R
通讯作者：
Borriss R
影响因子：
1.4
作者：
Apprill, Amy;McNally, Sean;Weber, Laura
通讯作者：
Weber, Laura
影响因子：
9.8
作者：
Bordenstein SR;Theis KR
通讯作者：
Theis KR