Identification of a Hormonally Regulated Luteinizing Hormone/Human Chorionic Gonadotropin Receptor mRNA Binding Protein
Identification of a Hormonally Regulated Luteinizing Hormone/Human Chorionic Gonadotropin Receptor mRNA Binding Protein
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激素调节的黄体生成激素/人绒毛膜促性腺激素受体 mRNA 结合蛋白的鉴定
DOI：
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发表时间：
1998
影响因子：
4.8
通讯作者：
K. Menon
中科院分区：
文献类型：
--
作者：
J. Kash;K. Menon
为了阐明在hCG诱导的受体下调过程中与黄体生成素/人绒毛膜促性腺激素（LH/hCG）受体mRNA稳定性调节相关的分子事件，我们鉴定了LH/hCG受体特异性mRNA结合蛋白。从对照和下调的大鼠卵巢中分离蛋白质，并与对应于全长LH/hCG受体以及5′-和3′-截短受体形式的体外转录RNA孵育。通过RNA凝胶迁移率变动分析所得核糖核蛋白复合物。一个突出的M r 50，000核糖核蛋白复合物被鉴定具有以下特征：1）对位于核苷酸102和282之间的LH/hCG受体开放阅读框序列的特异性; 2）缺乏非特异性RNA的竞争; 3）在hCG诱导的受体下调期间RNA结合活性增加3倍;和4）有限的组织表达。这份报告描述了第一个证据的LH/hCG受体mRNA结合蛋白，我们称之为LRBP-1，促黄体激素受体RNA结合蛋白-1。该蛋白是参与大鼠卵巢LH/hCG受体mRNA稳定性的激素调节的反式作用因子的候选者。
To elucidate the molecular events associated with the regulation of luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor mRNA stability during hCG-induced receptor down-regulation, we have identified an LH/hCG receptor-specific mRNA binding protein. Proteins were isolated from control and down-regulated rat ovary and were incubated with in vitrotranscribed RNAs corresponding to the full-length LH/hCG receptor, as well as 5′- and 3′-truncated receptor forms. Resultant ribonucleoprotein complexes were analyzed by RNA gel mobility shift. A prominent M r 50,000 ribonucleoprotein complex was identified with the following characteristics: 1) specificity for LH/hCG receptor open reading frame sequences located between nucleotides 102 and 282; 2) lack of competition by nonspecific RNAs; 3) a 3-fold increase in RNA binding activity during hCG-induced receptor down-regulation; and 4) limited tissue expression. This report describes the first evidence of an LH/hCG receptor mRNA binding protein, which we term LRBP-1, for luteinizing hormone receptor RNA binding protein-1. This protein is a candidate for atrans-acting factor involved in the hormonal regulation of LH/hCG receptor mRNA stability in rat ovary.
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3非翻译区介导的黄体生成激素/人绒毛膜促性腺激素受体表达的调节。
DOI：
10.1021/bi961019a
发表时间：
1996
期刊：
Biochemistry.
影响因子：
--
作者：
Lu,DL;Menon,KM
通讯作者：
Menon,KM
铁调节元件 (IRE)：mRNA 非编码序列家族。
DOI：
10.1042/bj3040001
发表时间：
1994
期刊：
The Biochemical journal
影响因子：
--
作者：
Theil,EC
通讯作者：
Theil,EC
影响因子：
10.5
作者：
BERNSTEIN, PL;HERRICK, DJ;ROSS, J
通讯作者：
ROSS, J
c-myc mRNA 的蛋白质编码区包含指定快速 mRNA 周转和蛋白质合成抑制剂诱导的序列。
影响因子：
10.5
作者：
Wisdom,R;Lee,W
通讯作者：
Lee,W
多种黄体生成素/绒毛膜促性腺激素受体信使核糖核酸转录物。
影响因子：
4.8
作者：
Wang,H;Ascoli,M;Segaloff,DL
通讯作者：
Segaloff,DL