Two-photon and second harmonic microscopy in clinical and translational cancer research.
Two-photon and second harmonic microscopy in clinical and translational cancer research.
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DOI：
10.1007/s10439-012-0512-9
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发表时间：
2012-02
影响因子：
3.8
通讯作者：
Brown, Edward B.
中科院分区：
文献类型：
--
作者：
Perry, Seth W.;Burke, Ryan M.;Brown, Edward B.
在过去的几年里，随着临床前研究的几个途径取得成果，双光子显微镜（TPM）在转化和临床癌症研究中的应用迅速发展。在这篇综述中，我们重点介绍了两种形式的tpm -双光子激发荧光显微镜和二次谐波产生显微镜-因为它们已被用于研究离体和体内人体组织的癌症病理。我们首先讨论双光子理论和仪器，特别是适用于癌症研究的双光子理论和仪器，然后概述一些相关的癌症研究文献，包括人体组织活检的双光子成像、体内人体皮肤和快速发展的双光子显微内窥镜技术。我们相信这些和其他不断发展的双光子方法将继续帮助将癌症研究从实验室转化为床边，并最终将癌症诊断和治疗的微创方法变为现实。
Application of two-photon microscopy (TPM) to translational and clinical cancer research has burgeoned over the last several years, as several avenues of pre-clinical research have come to fruition. In this review, we focus on two forms of TPM—two-photon excitation fluorescence microscopy, and second harmonic generation microscopy—as they have been used for investigating cancer pathology in ex vivo and in vivo human tissue. We begin with discussion of two-photon theory and instrumentation particularly as applicable to cancer research, followed by an overview of some of the relevant cancer research literature in areas that include two-photon imaging of human tissue biopsies, human skin in vivo, and the rapidly developing technology of two-photon microendoscopy. We believe these and other evolving two-photon methodologies will continue to help translate cancer research from the bench to the bedside, and ultimately bring minimally invasive methods for cancer diagnosis and treatment to therapeutic reality.
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影响因子：
6
作者：
Conklin, Matthew W.;Eickhoff, Jens C.;Keely, Patricia J.
通讯作者：
Keely, Patricia J.
影响因子：
3.5
作者：
Chang, Yu-Chung;Ye, Jing Yong;Norris, Theodore B.
通讯作者：
Norris, Theodore B.
影响因子：
3.5
作者：
Bao, Hongchun;Boussioutas, Alex;Gu, Min
通讯作者：
Gu, Min
影响因子：
1.2
作者：
Ammer, Amanda Gatesman;Hayes, Karen E.;Weed, Scott A.
通讯作者：
Weed, Scott A.
在组织病理学切片中内源性荧光团的荧光寿命成像揭示了乳腺癌原位癌中正常和肿瘤上皮之间的差异。
影响因子：
2.6
作者：
Conklin MW;Provenzano PP;Eliceiri KW;Sullivan R;Keely PJ
通讯作者：
Keely PJ