The Role of Saliva and Serum in Candida albicans Biofilm Formation on Denture Acrylic Surfaces
The Role of Saliva and Serum in Candida albicans Biofilm Formation on Denture Acrylic Surfaces
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唾液和血清在假牙亚克力表面白色念珠菌生物膜形成中的作用
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
L. Samaranayake
中科院分区：
文献类型：
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作者：
H. Nikawa;H. Nishimura;T. Yamamoto;T. Hamada;L. Samaranayake
唾液或血清薄膜对义齿丙烯酸表面上白色念珠菌生物膜形成的长期影响通过定量所得生物膜的ATP（三磷酸腺苷）含量和扫描电子显微镜进行了研究。当检查唾液涂覆的丙烯酸条上的生物膜形成时，酵母最初以比对照慢的速率定殖该表面，尽管随着孵育时间的增加，在72小时时，ATP含量几乎是无蛋白对照条的10倍。超微结构研究表明，这是由于细胞聚集和菌丝出现，在对照中未观察到的现象。与对照条相比，生物膜活性的血清包被的条是几乎100倍，在48小时的孵育，和扫描电子显微镜显示多层芽孢子-芽孢子共粘附，芽管，菌丝和假菌丝的出现和芽孢子-菌丝共粘附。进一步的免疫细胞化学观察显示，伴刀豆球蛋白-A结合材料和纤连蛋白参与唾液和血清包被标本上的生物膜形成，此外，甘露聚糖结合蛋白和蛋白-A结合材料也有助于血清包被标本上的生物膜形成。关键词：白色念珠菌，生物膜，唾液，血清，纤维连接蛋白，甘露聚糖结合蛋白。
The long term effect of either a salivary or a serum pellicle on Candida albicans biofilm formation on denture acrylic surfaces was investigated both by quantifying the ATP (adenosine triphosphate) content of the resultant biofilms and by scanning electron microscopy. When the biofilm formation on saliva-coated acrylic strips was examined, the yeasts initially colonised this surface at a slower rate than the controls although with increasing incubation time, at 72 h, the ATP content was almost ten-fold higher than the protein-free control strips. Ultrastructural studies revealed this to be due to cell aggregation and hyphal emergence, phenomena not observed in the controls. As compared with the control strips, biofilm activity of the serum-coated strips was almost 100-fold greater within 48 h incubation, and scanning electron microscopy revealed multilayer blastospore-blastospore co-adhesion, germ tube, hyphal and pseudohyphal emergence and blastospore-hyphal coadherence. Further immunocytochemical observation revealed that concanavalin-A binding material and fibronectin were involved in biofilm formation on both saliva and serum coated specimens and, in addition, mannan-binding protein and protein-A binding material also contributed to the biofilm formation on serum coated specimens. Keywords: Candida albicans , biofilm, saliva, serum, fibronectin, mannan-binding protein.
念珠菌物种对宿主组织和塑料表面的粘附。
DOI：
10.1093/clinids/8.1.73
发表时间：
1986
期刊：
Reviews of infectious diseases
影响因子：
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作者：
Rotrosen,D;Calderone,RA;EdwardsJr,JE
通讯作者：
EdwardsJr,JE