Anti-fibrosis activity of combination therapy with epigallocatechin gallate, taurine and genistein by regulating glycolysis, gluconeogenesis, and ribosomal and lysosomal signaling pathways in HSC-T6 cells
Anti-fibrosis activity of combination therapy with epigallocatechin gallate, taurine and genistein by regulating glycolysis, gluconeogenesis, and ribosomal and lysosomal signaling pathways in HSC-T6 cells
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表没食子儿茶素没食子酸酯、牛磺酸和金雀异黄素联合治疗通过调节 HSC-T6 细胞中的糖酵解、糖异生以及核糖体和溶酶体信号通路的抗纤维化活性
DOI：
10.3892/etm.2018.6743
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发表时间：
2018-09
影响因子：
2.7
通讯作者：
Liao Ming
中科院分区：
文献类型：
--
作者：
Li Yan;Zhu Min;Huo Yani;Zhang Xuerong;Liao Ming
我们小组先前的一项研究表明，牛磺酸、表没食子儿茶素没食子酸酯（EGCG）和染料木黄酮联合治疗可预防肝纤维化。本研究的目的是阐明这种联合治疗的抗纤维化机制。
A previous study by our group indicated that combined treatment with taurine, epigallocatechin gallate (EGCG) and genistein protects against liver fibrosis. The aim of the present study was to elucidate the antifibrotic mechanism of this combination treatment using isobaric tag for relative and absolute quantification (iTRAQ)-based proteomics in an activated rat hepatic stellate cell (HSC) line. In the present study, HSC-T6 cells were incubated with taurine, EGCG and genistein, and cellular proteins were extracted and processed for iTRAQ labeling. Quantification and identification of proteins was performed using two-dimensional liquid chromatography coupled with tandem mass spectrometry. Proteomic analysis indicated that the expression of 166 proteins were significantly altered in response to combination treatment with taurine, EGCG and genistein. A total 76 of these proteins were upregulated and 90 were downregulated. Differentially expressed proteins were grouped according to their association with specific Kyoto Encyclopedia of Genes and Genomes pathways. The results indicated that the differentially expressed proteins hexokinase-2 and lysosome-associated membrane glycoprotein 1 were associated with glycolysis, gluconeogenesis and lysosome signaling pathways. The expression of these proteins was validated using western blot analysis; the expression of hexokinase-2 was significantly decreased and the expression of lysosome-associated membrane glycoprotein 1 was significantly increased in HSC-T6 cells treated with taurine, EGCG and genistein compared with the control, respectively (P<0.05). These results were in accordance with the changes in protein expression identified using the iTRAQ approach. Therefore, the antifibrotic effect of combined therapy with taurine, EGCG and genistein may be associated with the activation of several pathways in HSCs, including glycolysis, gluconeogenesis, and the ribosome and lysosome signaling pathways. The differentially expressed proteins identified in the current study may be useful for treatment of liver fibrosis in the future.
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Txn1、Ctsd 和 Cdk4 是牛磺酸、表没食子儿茶素没食子酸酯和金雀异黄素联合治疗大鼠肝纤维化的关键蛋白
影响因子：
7.5
作者：
Cao, Wen;Li, Yan;Liao, Ming
通讯作者：
Liao, Ming
DOI：
10.4103/1319-3767.96445
发表时间：
2012-05
期刊：
Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association
影响因子：
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作者：
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通讯作者：
Ahmad R
牛磺酸、表没食子儿茶素没食子酸酯和金雀异黄素联合治疗可减少 HSC-T6 细胞增殖并调节纤维形成因子的表达。
影响因子：
5.6
作者：
Li Y;Luo Y;Zhang X;Lin X;He M;Liao M
通讯作者：
Liao M
影响因子：
7.1
作者：
Sookoian, Silvia;Castano, Gustavo O.;Pirola, Carlos J.
通讯作者：
Pirola, Carlos J.
DOI：
10.12122/j.issn.1673-4254.2019.05.10
发表时间：
2019-05
影响因子：
--
作者：
Xipeng Liu;Mei-fang Zhang;Hai-feng Zhang;An-da Zhao;Juan Sun;Wen Tang
通讯作者：
Xipeng Liu;Mei-fang Zhang;Hai-feng Zhang;An-da Zhao;Juan Sun;Wen Tang