Discrimination of p53 immunohistochemistry-positive tumors by its staining pattern in gastric cancer.
Discrimination of p53 immunohistochemistry-positive tumors by its staining pattern in gastric cancer.
复制标题
通过胃癌染色模式来区分p53免疫组织化学阳性肿瘤。
DOI：
10.1002/cam4.346
复制
发表时间：
2015-01
期刊：
影响因子：
4
通讯作者：
Maehara, Yoshihiko
中科院分区：
文献类型：
--
作者：
Ando, Koji;Oki, Eiji;Saeki, Hiroshi;Yan, Zhao;Tsuda, Yasuo;Hidaka, Gen;Kasagi, Yuta;Otsu, Hajime;Kawano, Hiroyuki;Kitao, Hiroyuki;Morita, Masaru;Maehara, Yoshihiko
p53免疫组化染色是检测p53功能异常的一种廉价、简便的方法。然而，免疫组化染色结果与突变分析结果之间存在一些差异。本研究试图从p53的染色模式出发，对p53的染色进行新的定义。对148例胃癌患者进行了p53免疫组织化学染色和TP53基因突变分析。并对4例患者进行SNP-CGH阵列分析。p53蛋白在88例（59.5%）肿瘤中呈阳性表达。与阴性肿瘤相比，p53染色阳性的肿瘤显示恶性特征。TP 53基因突变发生率为19.6%，且年龄和分化程度较高。在p53阳性的肿瘤中，可分为两种类型：异常型和分散型。与TP53基因突变分析比较，散在型均存在野生型TP53基因（P = 0.0003）。SNP-CGH阵列显示散在型肿瘤17号染色体结构无改变。P53散在型染色肿瘤可能反映了功能活跃的非突变TP53基因。在p53免疫组化染色的解释，区分p53阳性肿瘤的染色模式可能是重要的胃癌。
Immunohistochemistry staining of p53 is a cheap and simple method to detect aberrant function of p53. However, there are some discrepancies between the result of immunohistochemistry staining and mutation analysis. This study attempted to find a new definition of p53 staining by its staining pattern. Immunohistochemistry staining of p53 and TP53 gene mutation analysis were performed in 148 gastric cancer patients. Also SNP-CGH array analysis was conducted to four cases. Positive staining of p53 was observed in 88 (59.5%) tumors. Tumors with positive p53 staining showed malignant features compared to negative tumors. Mutation of TP53 gene was observed in 29 (19.6%) tumors with higher age and differentiated type. In positive p53 tumors, two types could be distinguished; aberrant type and scattered type. With comparison to TP53 gene mutation analysis, all the scattered type had wild-type TP53 gene (P = 0.0003). SNP-CGH array showed that scattered-type tumors had no change in the structure of chromosome 17. P53-scattered-type staining tumors may reflect a functionally active nonmutated TP53 gene. In interpretation of p53 immunohistochemistry staining, distinguishing p53-positive tumors by their staining pattern may be important in gastric cancer.
登录
查看更多内容
影响因子：
3.9
作者：
Olivier, M;Eeles, R;Hainaut, P
通讯作者：
Hainaut, P
影响因子：
4.6
作者：
BURNS, JS;BLAYDES, JP;WYNFORDTHOMAS, D
通讯作者：
WYNFORDTHOMAS, D
影响因子：
5.7
作者：
Egashira, Akinori;Morita, Masaru;Maehara, Yoshihiko
通讯作者：
Maehara, Yoshihiko
影响因子：
7.3
作者：
BAAS, IO;MULDER, JWR;HAMILTON, SR
通讯作者：
HAMILTON, SR
影响因子：
7.4
作者：
通讯作者：
--