Transcription Factor AsMYC2 Controls the Jasmonate-Responsive Expression of ASS1 Regulating Sesquiterpene Biosynthesis in Aquilaria sinensis (Lour.) Gilg
Transcription Factor AsMYC2 Controls the Jasmonate-Responsive Expression of ASS1 Regulating Sesquiterpene Biosynthesis in Aquilaria sinensis (Lour.) Gilg
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转录因子 AsMYC2 控制 ASS1 的茉莉酸响应性表达，调节白木香倍半萜生物合成
DOI：
10.1093/pcp/pcx122
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Jian-He Wei
中科院分区：
文献类型：
--
作者：
Yan-Hong Xu;Yong-Cui Liao;Fei-Fei Lv;Zheng Zhang;Pei-Wen Sun;Zhi-Hui Gao;Ke-Ping Hu;Chun Sui;Yue Jin;Jian-He Wei
倍半萜类化合物是沉香中最重要的防御性次生代谢成分之一。沉香是沉香对外界伤害的反应产物，是一种芳香的木材，广泛用于传统药物，熏香和香水。我们以前报道过茉莉酸（jasmonic acid，JA）在促进沉香倍半萜合成中起重要作用，并诱导沉香倍半萜合成酶ASS 1的表达，该酶是沉香倍半萜合成的关键酶。sinensis。然而，人们对这种分子调控机制知之甚少。在此，我们鉴定了来自A.作为ASS 1表达的激活剂。AsMYC2是一个立即早期茉莉酸响应基因，与ASS 1共诱导。使用酵母单杂交试验和染色质免疫沉淀分析的组合，我们表明，AsMYC2绑定的启动子的ASS 1含有G盒基序。AsMYC2激活烟草表皮细胞中ASS 1的表达，上调拟南芥中倍半萜合酶基因（TPS21和TPS11）的表达，茉莉酸甲酯也能促进其表达。我们的研究结果表明AsMYC2参与了沉香倍半萜生物合成的调控。通过JA信号通路控制ASS 1的表达，从而抑制了AS 1的表达。
Sesquiterpenes are one of the most important defensive secondary metabolite components of agarwood. Agarwood, which is a product of the Aquilaria sinensis response to external damage, is a fragrant and resinous wood that is widely used in traditional medicines, incense and perfume. We previously reported that jasmonic acid (JA) plays an important role in promoting agarwood sesquiterpene biosynthesis and induces expression of the sesquiterpene synthase ASS1, which is a key enzyme that is responsible for the biosynthesis of agarwood sesquiterpenes in A. sinensis. However, little is known about this molecular regulation mechanism. Here, we characterized a basic helix-loop-helix transcription factor, AsMYC2, from A. sinensis as an activator of ASS1 expression. AsMYC2 is an immediate-early jasmonate-responsive gene and is co-induced with ASS1. Using a combination of yeast one-hybrid assays and chromatin immunoprecipitation analyses, we showed that AsMYC2 bound the promoter of ASS1 containing a G-box motif. AsMYC2 activated expression of ASS1 in tobacco epidermis cells and up-regulated expression of sesquiterpene synthase genes (TPS21 and TPS11) in Arabidopsis, which was also promoted by methyl jasmonate. Our results suggest that AsMYC2 participates in the regulation of agarwood sesquiterpene biosynthesis in A. sinensis by controlling the expression of ASS1 through the JA signaling pathway.
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影响因子：
1.8
作者：
Hedhili S;De Mattei MV;Coudert Y;Bourrié I;Bigot Y;Gantet P
通讯作者：
Gantet P
沉香形成相关基因的鉴定：沉香健康和受伤组织的转录组分析。
影响因子：
4.4
作者：
Xu Y;Zhang Z;Wang M;Wei J;Chen H;Gao Z;Sui C;Luo H;Zhang X;Yang Y;Meng H;Li W
通讯作者：
Li W
影响因子：
--
作者：
Jaideep Chaudhary;Michael K. Skinner
通讯作者：
Jaideep Chaudhary;Michael K. Skinner
影响因子：
4.6
作者：
Ogawa S;Miyamoto K;Nemoto K;Sawasaki T;Yamane H;Nojiri H;Okada K
通讯作者：
Okada K
影响因子：
1.7
作者：
K. Hashimoto;Sachiko Nakahara;Takehisa Inoue;Y. Sumida;Mutsuko Takahashi;Y. Masada
通讯作者：
K. Hashimoto;Sachiko Nakahara;Takehisa Inoue;Y. Sumida;Mutsuko Takahashi;Y. Masada