OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability.
OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability.
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DOI：
10.1038/srep40175
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发表时间：
2017-01-09
影响因子：
4.6
通讯作者：
Okada K
中科院分区：
文献类型：
--
作者：
Ogawa S;Miyamoto K;Nemoto K;Sawasaki T;Yamane H;Nojiri H;Okada K
樱花素是一种存在于水稻中的类黄酮抗真菌植物防御素，其生物合成高度依赖于茉莉酸（JA）信号传导并受多种环境刺激诱导。我们先前鉴定了OsNOMT，其编码柚皮素7-O-甲基转移酶（NOMT）; NOMT是樱花素生产的关键酶。虽然OsNOMT的表达是由JA处理诱导的，但激活樱花醇生物合成途径的调节机制尚未阐明。在这项研究中，我们表明，JA诱导的碱性螺旋-环-螺旋转录因子OsMYC 2大大提高了OsNOMT启动子的活性，是必不可少的JA诱导樱花生产。此外，我们鉴定了OsMYC 2的2个合作者，OsMYC 2样蛋白1和2（OsMYL 1和OsMYL 2），其进一步与OsMYC 2协同激活OsNOMT启动子。OsMYC 2与OsMYL 1和OsMYL 2的物理相互作用进一步支持了这些相互作用导致OsMYC 2的反式激活活性增强的想法。我们的研究结果表明，JA信号通过OsMYC 2被OsMYL 1和OsMYL 2加强，导致在水稻防御反应中诱导产生樱花素。
Biosynthesis of sakuranetin, a flavonoid anti-fungal phytoalexin that occurs in rice, is highly dependent on jasmonic acid (JA) signalling and induced by a variety of environmental stimuli. We previously identified OsNOMT, which encodes naringenin 7-O-methyltransferase (NOMT); NOMT is a key enzyme for sakuranetin production. Although OsNOMT expression is induced by JA treatment, the regulation mechanism that activates the biosynthetic pathway of sakuranetin has not yet been elucidated. In this study, we show that JA-inducible basic helix-loop-helix transcriptional factor OsMYC2 drastically enhances the activity of the OsNOMT promoter and is essential for JA-inducible sakuranetin production. In addition, we identified 2 collaborators of OsMYC2, OsMYC2-like protein 1 and 2 (OsMYL1 and OsMYL2) that further activated the OsNOMT promoter in synergy with OsMYC2. Physical interaction of OsMYC2 with OsMYL1 and OsMYL2 further supported the idea that these interactions lead to the enhancement of the transactivation activity of OsMYC2. Our results indicate that JA signalling via OsMYC2 is reinforced by OsMYL1 and OsMYL2, resulting in the inductive production of sakuranetin during defence responses in rice.
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