Structural Biology of Nanobodies against the Spike Protein of SARS-CoV-2.
Structural Biology of Nanobodies against the Spike Protein of SARS-CoV-2.
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DOI：
10.3390/v13112214
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发表时间：
2021-11-03
期刊：
影响因子：
--
通讯作者：
Naismith JH
中科院分区：
文献类型：
--
作者：
Tang Q;Owens RJ;Naismith JH
纳米抗体是130个氨基酸的单结构域抗体（VHH），其来源于骆驼免疫球蛋白的独特的仅重链亚类。它们的小分子尺寸，易于表达，高亲和力和稳定性相结合，使其成为独特的靶向试剂，在生物医学科学中具有众多应用。第一个纳米抗体制剂现在已经进入临床，作为对血液疾病的治疗。SARS-CoV-2病毒的传播使全球科学界努力加速技术的发展，以试图击败目前已造成400多万人死亡的大流行病。在非常短的时间内，多项研究报告了针对病毒刺突蛋白的纳米抗体。已在培养物中检测了几种试剂，并证明对病毒或假病毒具有强效中和作用。一些药剂已经完成了动物试验，结果非常令人鼓舞，显示了它们治疗感染的潜力。在这里，我们讨论的结构特征，指导纳米抗体识别的刺突蛋白的受体结合域的SARS-CoV-2。
Nanobodies are 130 amino acid single-domain antibodies (VHH) derived from the unique heavy-chain-only subclass of Camelid immunogloblins. Their small molecular size, facile expression, high affinity and stability have combined to make them unique targeting reagents with numerous applications in the biomedical sciences. The first nanobody agent has now entered the clinic as a treatment against a blood disorder. The spread of the SARS-CoV-2 virus has seen the global scientific endeavour work to accelerate the development of technologies to try to defeat a pandemic that has now killed over four million people. In a remarkably short period of time, multiple studies have reported nanobodies directed against the viral Spike protein. Several agents have been tested in culture and demonstrate potent neutralisation of the virus or pseudovirus. A few agents have completed animal trials with very encouraging results showing their potential for treating infection. Here, we discuss the structural features that guide the nanobody recognition of the receptor binding domain of the Spike protein of SARS-CoV-2.
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影响因子：
16.6
作者：
Greaney AJ;Starr TN;Barnes CO;Weisblum Y;Schmidt F;Caskey M;Gaebler C;Cho A;Agudelo M;Finkin S;Wang Z;Poston D;Muecksch F;Hatziioannou T;Bieniasz PD;Robbiani DF;Nussenzweig MC;Bjorkman PJ;Bloom JD
通讯作者：
Bloom JD
DOI：
10.1083/jcb.201409074
发表时间：
2015-06-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Helma J;Cardoso MC;Muyldermans S;Leonhardt H
通讯作者：
Leonhardt H
影响因子：
64.8
作者：
Barnes CO;Jette CA;Abernathy ME;Dam KA;Esswein SR;Gristick HB;Malyutin AG;Sharaf NG;Huey-Tubman KE;Lee YE;Robbiani DF;Nussenzweig MC;West AP Jr;Bjorkman PJ
通讯作者：
Bjorkman PJ
影响因子：
3.5
作者：
Girt GC;Lakshminarayanan A;Huo J;Dormon J;Norman C;Afrough B;Harding A;James W;Owens RJ;Naismith JH
通讯作者：
Naismith JH
影响因子：
16.8
作者：
Huo, Jiangdong;Le Bas, Audrey;Naismith, James H.
通讯作者：
Naismith, James H.