Pervasive acquisition of CRISPR memory driven by inter-species mating of archaea can limit gene transfer and influence speciation.
Pervasive acquisition of CRISPR memory driven by inter-species mating of archaea can limit gene transfer and influence speciation.
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DOI：
10.1038/s41564-018-0302-8
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发表时间：
2019-01
影响因子：
28.3
通讯作者：
Gophna U
中科院分区：
文献类型：
--
作者：
Turgeman-Grott I;Joseph S;Marton S;Eizenshtein K;Naor A;Soucy SM;Stachler AE;Shalev Y;Zarkor M;Reshef L;Altman-Price N;Marchfelder A;Gophna U
CRISPR-Cas系统为原核生物提供了针对病毒和质粒的序列特异性免疫力，基于从这些入侵者（称为间隔区）获得的DNA。令人惊讶的是，许多古细菌拥有与相关物种染色体基因相匹配的间隔区，包括编码核心管家基因的间隔区。通过对从单个站点分离的环境古细菌的基因组进行测序，我们证明了种间间隔区是常见的。我们表明实验交配Haloferax volcano ii和Haloferax mediomei，间隔区确实收购染色体范围内，虽然优先集成移动的元素和附近地区的染色体存在。种间交配诱导增加间隔区收购，并可能导致两个物种的收购机制之间的相互作用。令人惊讶的是，许多间隔区收购后，物种间交配目标自我复制沿着那些来自交配伴侣，表明收购机制不能区分自我非自我在这些条件下。将一个物种的染色体改造成另一个物种的CRISPR-Cas靶向，大大减少了它们之间的基因交换。因此，在物种间交配期间获得的间隔区可能会限制未来的基因转移，从而导致CRISPR-Cas系统在微生物物种形成中发挥作用。
CRISPR-Cas systems provide prokaryotes with sequence-specific immunity against viruses and plasmids, based on DNA acquired from these invaders, known as spacers. Surprisingly, many archaea possess spacers that match chromosomal genes of related species, including those encoding core housekeeping genes. By sequencing genomes of environmental archaea isolated from a single site, we demonstrate that inter-species spacers are common. We show experimentally by mating Haloferax volcanii and Haloferax mediterranei, that spacers are indeed acquired chromosome-wide, although a preference for integrated mobile elements and nearby regions of the chromosome exists. Inter-species mating induces increased spacer acquisition and may result in interactions between the acquisition machinery of the two species. Surprisingly, many of the spacers acquired following inter-species mating target self-replicons along with those originating from the mating partner, indicating that the acquisition machinery cannot distinguish self from non-self under these conditions. Engineering the chromosome of one species to be targeted by the other's CRISPR-Cas reduces gene exchange between them substantially. Thus, spacers acquired during inter-species mating could limit future gene transfer, resulting in a role for CRISPR-Cas systems in microbial speciation.
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影响因子：
16
作者：
Leenay RT;Maksimchuk KR;Slotkowski RA;Agrawal RN;Gomaa AA;Briner AE;Barrangou R;Beisel CL
通讯作者：
Beisel CL
影响因子：
5.5
作者：
Brodt A;Lurie-Weinberger MN;Gophna U
通讯作者：
Gophna U
影响因子：
64.8
作者：
Levy A;Goren MG;Yosef I;Auster O;Manor M;Amitai G;Edgar R;Qimron U;Sorek R
通讯作者：
Sorek R
影响因子：
3.2
作者：
Deveau, Helene;Barrangou, Rodolphe;Moineau, Sylvain
通讯作者：
Moineau, Sylvain
代谢多功能嗜盐古菌 Haloferax mediterranei（一种聚（3-羟基丁酸酯-co-3-羟基戊酸酯）生产者）的完整基因组序列
影响因子：
3.2
作者：
Han, Jing;Zhang, Fan;Xiang, Hua
通讯作者：
Xiang, Hua