CRISPR loci reveal networks of gene exchange in archaea.
CRISPR loci reveal networks of gene exchange in archaea.
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DOI：
10.1186/1745-6150-6-65
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发表时间：
2011-12-21
期刊：
影响因子：
5.5
通讯作者：
Gophna U
中科院分区：
文献类型：
--
作者：
Brodt A;Lurie-Weinberger MN;Gophna U
CRISPR基因座（聚集、规则、间隔、短、回文重复）为原核生物提供了一种针对病毒和其他可移动遗传元件的适应性免疫。CRISPR阵列可以被转录并加工成小的crRNA分子，然后被细胞用来靶向外来核酸。由于间隔序列是由活跃的CRISPR/Cas系统积累的，因此这些间隔序列提供了生物体过去“感染史”的记录。在这里，我们分析了所有目前已知的古菌基因组中存在的间隔序列，并通过DNA相似性确定了它们的来源。虽然近50%的古细菌间隔与可移动的遗传元素（如质粒或病毒）相匹配，但其他一些间隔与其他生物体（主要是其他古细菌）的染色体基因相匹配。因此，通过间隔分析揭示了古细菌物种之间的基因交换网络，包括许多属间和种间的基因转移事件。识别病毒序列的间隔序列往往位于离先导序列较远的位置，这意味着它们的保留存在选择压力。CRISPR间隔片段为古细菌中广泛的基因交换提供了直接证据，特别是在属内，并支持了CRISPR/Cas系统的主要作用是抗病毒和抗质粒防御的当前教条。这篇文章由以下几位教授审阅。W. Ford Doolittle, John van der Oost, Christa Schleper（由董事会成员Prof. J Peter Gogarten提名）
CRISPR (Clustered, Regularly, Interspaced, Short, Palindromic Repeats) loci provide prokaryotes with an adaptive immunity against viruses and other mobile genetic elements. CRISPR arrays can be transcribed and processed into small crRNA molecules, which are then used by the cell to target the foreign nucleic acid. Since spacers are accumulated by active CRISPR/Cas systems, the sequences of these spacers provide a record of the past "infection history" of the organism. Here we analyzed all currently known spacers present in archaeal genomes and identified their source by DNA similarity. While nearly 50% of archaeal spacers matched mobile genetic elements, such as plasmids or viruses, several others matched chromosomal genes of other organisms, primarily other archaea. Thus, networks of gene exchange between archaeal species were revealed by the spacer analysis, including many cases of inter-genus and inter-species gene transfer events. Spacers that recognize viral sequences tend to be located further away from the leader sequence, implying that there exists a selective pressure for their retention. CRISPR spacers provide direct evidence for extensive gene exchange in archaea, especially within genera, and support the current dogma where the primary role of the CRISPR/Cas system is anti-viral and anti-plasmid defense. This article was reviewed by: Profs. W. Ford Doolittle, John van der Oost, Christa Schleper (nominated by board member Prof. J Peter Gogarten)
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影响因子：
3.1
作者：
REDFIELD, RJ
通讯作者：
REDFIELD, RJ
DOI：
10.1038/nrg2749
发表时间：
2010-03
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.2
作者：
Anderson, Rika E.;Brazelton, William J.;Baross, John A.
通讯作者：
Baross, John A.
影响因子：
5.6
作者：
Prangishvili, David;Vestergaard, Gisle;Garrett, Roger A.
通讯作者：
Garrett, Roger A.
DOI：
10.1126/science.1192272
发表时间：
2010-09-10
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Haurwitz RE;Jinek M;Wiedenheft B;Zhou K;Doudna JA
通讯作者：
Doudna JA